L4402

Lysostaphin aus Staphylococcus staphylolyticus

BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

• Synonym(e): Glycyl-glycine Endopeptidase
• CAS-Nummer: 9011-93-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• Specific activity: ≥2,000 units/mg protein
• Assay: ≥97% (SDS-PAGE)
• Biological source: bacterial (Staphylococcus sp.)
• Concentration: 40—60% protein

Eigenschaften

• biological source: bacterial (Staphylococcus sp.)
• Quality Segment: 300
• product line: BioUltra
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥2,000 units/mg protein
• mol wt: 25 kDa
• composition: Protein, 40-60% biuret
• concentration: 40—60% protein
• technique(s): cell based assay: suitable
• input: bacteria and yeast sample(s)
• suitability: suitable for cell lysis
• antibiotic activity spectrum: Gram-positive bacteria
• application(s): diagnostic assay manufacturing
• mode of action: cell wall synthesis | interferes
• storage temp.: −20°C

Beschreibung

General description

Chemical structure: peptide

Package size based on protein content

Biochem/physiol Actions

Lysostaphin ist eine Zink-Endopeptidase mit einer Molekülmasse von etwa 25 kDa. Da Lysostaphin die Polyglycinvernetzungen in der Peptidoglykanschicht der Zellwand von Staphylococcus-Spezies spaltet, ist es nützlich für die Zelllyse und auch als potenzielles antimikrobielles Therapeutikum.
Optimaler pH-Wert für Aktivität: ~7,5

Physical form

Contains potassium phosphate buffer salts and sodium chloride

Preparation Note

Affinity purified

Other Notes

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)