SAE0091

Lysostaphin aus Staphylococcus staphylolyticus

free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research

• Synonym(e): Glycyl-glycine Endopeptidase
• CAS-Nummer: 9011-93-2
• UNSPSC Code: 12352202
• NACRES: NA.54
• EG-Nummer: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556

Eigenschaften

• Produktname: Lysostaphin aus Staphylococcus staphylolyticus, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein
• biological source: microbial (Staphylococcus staphylotyticus)
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 27 kDa
• feature: DNA free
• input: bacteria and yeast sample(s)
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

Application

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

Biochem/physiol Actions

Lysostaphin ist eine Zink-Endopeptidase mit einer Molekülmasse von etwa 25 kDa. Da Lysostaphin die Polyglycinvernetzungen in der Peptidoglykanschicht der Zellwand von Staphylococcus-Spezies spaltet, ist es nützlich für die Zelllyse und auch als potenzielles antimikrobielles Therapeutikum.
Optimaler pH-Wert für Aktivität: ~7,5

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.

Physical form

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

Other Notes

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable