P4664

o-Phenylendiamin -dihydrochlorid

chromogenic, tablet

• Synonym(e): 1,2-Phenylenediamine tablet, OPD, OPD Tablet
• Lineare Formel: C₆H₄(NH₂)₂·2HCl
• CAS-Nummer: 615-28-1
• Molekulargewicht: 181.06
• NACRES: NA.83
• PubChem Substance ID: 24898491
• UNSPSC Code: 12352204
• MDL number: MFCD00012966
• Beilstein/REAXYS Number: 3912045

Eigenschaften

• Produktname: o-Phenylendiamin -dihydrochlorid, tablet, 15 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• technique(s): protein detection: suitable
• mp: 258 °C (dec.) (lit.)
• solubility: water: 1 tablet/10 mL, clear, colorless
• storage temp.: 2-8°C
• SMILES string: Cl[H].Cl[H].Nc1ccccc1N
• InChI: 1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
• InChI key: RIIWUGSYXOBDMC-UHFFFAOYSA-N

Beschreibung

General description

o-Phenylendiamindihydrochlorid (OPD) ist ein chromogenes Substrat, das in Enzyme-linked-Immunosorbent-Assay(ELISA)-Verfahren eingesetzt werden kann, da es Meerrettichperoxidasekonjugate nutzt. Das Substrat produziert ein lösliches Endprodukt mit orange-brauner Farbe, das bei 450 nm spektrofotometrisch gemessen werden kann. Die OPD-Reaktion kann mit 3 N HCl oder 3 mol/l H2SO4 gestoppt und bei 492 nm gemessen werden.

Application

o-Phenylenediamine dihydrochloride has been used:

• as a substrate in conventional capture enzyme-linked immunosorbent assay (ELISA) for recombinant hemagglutinin (rHA) protein detection
• as a substrate for the determination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin G (IgG) antibodies by ELISA
• as a colorimetric reagent specific for horseradish peroxidase (HRP) activity assessment in an ELISA protocol using SARS-CoV-2 antigens for S protein

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Sicherheitshinweise

• Piktogramme: GHS08,GHS07,GHS09
• Signalwort: Warning
• Gefahrencodes: H302 + H332,H317,H319,H341,H351,H410
• Sicherheitshinweise: P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges