P5538

Phosphoglucose-Isomerase aus Bacillus stearothermophilus

lyophilized powder, 300-1,000 units/mg protein

• Synonym(e): D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
• CAS-Nummer: 9001-41-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-603-1
• MDL number: MFCD00131887
• EG-Nummer: 5.3.1.9 (BRENDA, IUBMB)
• Specific activity: 300-1,000 units/mg protein

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• specific activity: 300-1,000 units/mg protein
• mol wt: 189 kDa
• composition: Protein, ≥60% biuret
• storage temp.: −20°C

Beschreibung

General description

The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa. Optimum pH is found to be between 9-10 and the isoelectric point is 4.2.

Application

The enzyme from Sigma has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti.

Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus.

Biochem/physiol Actions

Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.

Physical form

lyophilized powder containing Tris buffer

Other Notes

One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 9.0 at 30 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)