Technically, there is no limit to the percentage of water that can be used on a bare silica column. However, exposing the column to 100% water would require several hours to re-equilibrate back to typical HILIC conditions. Nevertheless, it is advisable to use 95% water and 5% Acetonitrile to avoid dewetting the phase, unless the column is explicitly labeled as aqueous compatible. After flushing the column sufficiently, a re-equilibration of no fewer than 75 column volumes is recommended.
581502-U
Ascentis® Si (3 µm) HPLC Columns
L × I.D. 15 cm × 2.1 mm, HPLC Column
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UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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Unterstützung erhaltenProduktname
Ascentis® Si HPLC-Säule, 3 μm particle size, L × I.D. 15 cm × 2.1 mm
material
stainless steel column
agency
suitable for USP L3
product line
Ascentis®
feature
endcapped: no
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
15 cm × 2.1 mm
surface area
450 m2/g
impurities
<5 ppm metals
matrix
fully porous particle
silica gel high purity, spherical
matrix active group
silica phase
particle size
3 μm
pore size
100 Å
operating pH range
2-6
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC)
normal phase
Quality Level
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Verwandte Kategorien
1 of 4
Dieser Artikel | 581514-U | 581513-U | 581503-U |
|---|---|---|---|
| particle size 3 μm | particle size 5 μm | particle size 5 μm | particle size 3 μm |
| matrix active group silica phase | matrix active group silica phase | matrix active group silica phase | matrix active group silica phase |
| L × I.D. 15 cm × 2.1 mm | L × I.D. 25 cm × 10 mm | L × I.D. 25 cm × 4.6 mm | L × I.D. 10 cm × 3 mm |
| separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase | separation technique hydrophilic interaction (HILIC), normal phase |
| agency suitable for USP L3 | agency suitable for USP L3 | agency suitable for USP L3 | agency suitable for USP L3 |
| product line Ascentis® | product line Ascentis® | product line Ascentis® | product line Ascentis® |
General description
Die Ascentis Si besitzt Kieselgel mit hoher Beladungskapazität und ausgezeichneten Peak-Form Eigenschaften. Die Ascentis Si ist sowohl für den Normal-Phase - als auch für den HILIC/ANP (wässrige Normalphase) Modus geeignet.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Deborah Benhaim et al.
Journal of chromatography. A, 1217(1), 65-74 (2009-11-27)
This study investigates lipophilicity determination by chromatographic measurements using the polar embedded Ascentis RP-Amide stationary phase. As a new generation of amide-functionalized silica stationary phase, the Ascentis RP-Amide column is evaluated as a possible substitution to the n-octanol/water partitioning system
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous
Shigehisa Uchiyama et al.
Analytical chemistry, 80(9), 3285-3290 (2008-03-18)
A new method for the simultaneous determination of ozone and carbonyls in air using a two-bed cartridge system has been developed. Each bed consists of reagent-impregnated silica particles. The first contains trans-1,2-bis-(4-pyridyl) ethylene (BPE) while the second contains 2,4-dinitrophenylhydrazine (DNPH).
Paweł Kubica et al.
Journal of chromatography. A, 1289, 13-18 (2013-04-04)
Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach
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What is the maximum percentage of water that should not be exceeded when using this column to prevent deactivation of the silica?
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