BPR-3P0128 inhibits RNA-dependent RNA polymerase elongation and VPg uridylylation activities of Enterovirus 71.

BPR-3P0128 inhibits RNA-dependent RNA polymerase elongation and VPg uridylylation activities of Enterovirus 71.

Antiviral research (2014-12-03)

Arul Balaji Velu, Guang-Wu Chen, Po-Ting Hsieh, Jim-Tong Horng, John Tsu-An Hsu, Hsing-Pang Hsieh, Tzu-Chun Chen, Kuo-Feng Weng, Shin-Ru Shih

PMID25448086

ZUSAMMENFASSUNG

Enterovirus 71 (EV71) infections can cause hand, foot, and mouth disease with severe neurological complications. Because no clinical drug is available for treating EV71 infections, developing an efficient antiviral medication against EV71 infection is crucial. This study indicated that 6-bromo-2-[1-(2,5-dimethylphenyl)-5-methyl-1H-pyrazol-4-yl] quinoline-4-carboxylic acid (BPR-3P0128) exhibits excellent antiviral activity against EV71 (EC50 = 0.0029 μM). BPR-3P0128 inhibits viral replication during the early post infection stage, targets EV71 RNA-dependent RNA polymerase and VPg uridylylation, and also reduces viral RNA accumulation levels and inhibits viral replication of EV71.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

L3771

Sigma-Aldrich

Natriumdodecylsulfat, BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)

75746

Sigma-Aldrich

Natriumdodecylsulfat, ≥99.0% (GC), dust-free pellets

S3014

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Natriumchlorid, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

S5150

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Natriumchlorid -Lösung, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

S7653

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Natriumchlorid, BioXtra, ≥99.5% (AT)

S8776

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Natriumchlorid -Lösung, 0.9% in water, BioXtra, suitable for cell culture

S5886

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Natriumchlorid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

436143

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Natriumdodecylsulfat, ACS reagent, ≥99.0%

71736

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Natriumdodecylsulfat -Lösung, BioUltra, Molecular Biology, 10% in H₂O

L4509

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Natriumdodecylsulfat, ReagentPlus^®, ≥98.5% (GC)

S6546

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Natriumchlorid -Lösung, 5 M

71725

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Natriumdodecylsulfat, BioUltra, Molecular Biology, ≥99.0% (GC)

05030

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Natriumdodecylsulfat -Lösung, BioUltra, 20% in H₂O

59222C

SAFC

Natriumchlorid -Lösung, 5 M

M0375

Sigma-Aldrich

Maleinsäure, ReagentPlus^®, ≥99% (HPLC)

74255

Supelco

Natriumdodecylsulfat, dust-free pellets, suitable for electrophoresis, Molecular Biology, ≥99.0% (GC)

P9625

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Phenazin-Methosulfat

71726

Supelco

Natriumdodecylsulfat, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

71376

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Natriumchlorid, BioUltra, Molecular Biology, ≥99.5% (AT)

S1679

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Natriumchlorid, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

71386

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Natriumchlorid -Lösung, BioUltra, Molecular Biology, ~5 M in H₂O

204439

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Natriumchlorid, 99.999% trace metals basis

PHR1321

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Natriumchlorid, Pharmaceutical Secondary Standard; Certified Reference Material

L6026

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Natriumdodecylsulfat, BioXtra, ≥99.0% (GC)

92816

Supelco

Maleinsäure, qNMR standard, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

71729

Sigma-Aldrich

Natriumdodecylsulfat, ≥98.0% (GC)

71387

Supelco

Natriumchlorid, reference material for titrimetry, certified by BAM, >99.5%

L5750

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Natriumdodecylsulfat, 92.5-100.5% based on total alkyl sulfate content basis

PHR1272

Supelco

Maleinsäure, Pharmaceutical Secondary Standard; Certified Reference Material

S6191

Sigma-Aldrich

Natriumchlorid, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture