A novel folic acid-conjugated TiO₂-SiO₂ photosensitizer for cancer targeting in photodynamic therapy.

A novel folic acid-conjugated TiO₂-SiO₂ photosensitizer for cancer targeting in photodynamic therapy.

Colloids and surfaces. B, Biointerfaces (2014-12-17)

Xiaohui Feng, Shaokun Zhang, Hong Wu, Xia Lou

ZUSAMMENFASSUNG

In this paper, a novel folic acid-conjugated silica-coated titanium dioxide (TiO2-SiO2) photosensitizer was synthesized and characterized using various analytical instruments. The photosensitizer was further assessed with regards to its photoreactivity, cellular and hemocompatibility, cell internalization, and phototoxicity. Conjugating folic acid with TiO2-SiO2 has shown a significantly improved compatibility of the nanoparticles with the mouse fibroblast cells (L929) at 24 h. An improved compatibility with the human nasopharyngeal epidermoid cancer (KB) cells was also demonstrated, but to a slightly reduced degree. Enhanced cell internalization was well demonstrated in the TiO2-SiO2 folate nanoparticles. Upon exposure to UV light, TiO2-SiO2 folate nanoparticles maintained a high level photodynamic reactivity and yielded a 38-43% photo-killing of KB cells. The photo-killing effect increased with increasing dosage in the investigated concentration range of 50-100 μg ml(-1).

MATERIALIEN

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Marke

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E7023

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Ethyl alcohol, Pure 200 proof, Molecular Biology

D2650

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Dimethylsulfoxid, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

472301

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Dimethylsulfoxid, ACS reagent, ≥99.9%

D8418

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Dimethylsulfoxid, Molecular Biology

34869

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Dimethylsulfoxid, suitable for HPLC, ≥99.7%

459844

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Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%

D2438

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Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

673811

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Diethylether, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor

S6014

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Natriumbicarbonat, ACS reagent, ≥99.7%

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Dimethylsulfoxid, ReagentPlus^®, ≥99.5%

276855

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Dimethylsulfoxid, anhydrous, ≥99.9%

493546

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Ethyl alcohol, Pure, 200 proof, meets USP testing specifications

309966

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Diethylether, suitable for HPLC, ≥99.9%, inhibitor-free

346136

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Diethylether, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor

223530

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Natriumcarbonat, powder, ≥99.5%, ACS reagent

222321

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Natriumcarbonat, ACS reagent, anhydrous, ≥99.5%, powder or granules

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440140

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