The Presence of Human Chorionic Gonadotropin/Luteinizing Hormone Receptors in Pancreatic β-Cells.

The Presence of Human Chorionic Gonadotropin/Luteinizing Hormone Receptors in Pancreatic β-Cells.

Reproductive sciences (Thousand Oaks, Calif.) (2015-02-12)

Jai Parkash, Zhenmin Lei, C V Rao

ZUSAMMENFASSUNG

We investigated the possible presence of functional human chorionic gonadotropin (hCG)/luteinizing hormone (LH) receptors in β-cells of pancreas, using a combination of techniques on hCG/LH receptor knockout mice, immortalized rat insulinoma cells, and human pancreatic islets. The results showed the presence of receptors and their activation resulted in a dose-dependent increase in glucose-induced release of insulin. These findings place hCG and LH among the regulators of insulin release with potential implications for insulin-level changes during the periods of altered hCG and LH secretion.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

G5516

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Glycerin, Molecular Biology, ≥99.0%

L3771

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Natriumdodecylsulfat, BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)

X100

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Triton^™ X-100, laboratory grade

75746

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Natriumdodecylsulfat, ≥99.0% (GC), dust-free pellets

43816

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DL-Dithiothreitol -Lösung, BioUltra, Molecular Biology, ~1 M in H₂O

S3014

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Natriumchlorid, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

S5150

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Natriumchlorid -Lösung, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

S7653

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Natriumchlorid, BioXtra, ≥99.5% (AT)

S5886

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Natriumchlorid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S8776

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Natriumchlorid -Lösung, 0.9% in water, BioXtra, suitable for cell culture

436143

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Natriumdodecylsulfat, ACS reagent, ≥99.0%

71736

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Natriumdodecylsulfat -Lösung, BioUltra, Molecular Biology, 10% in H₂O

646563

Supelco

DL-Dithiothreitol -Lösung, 1 M in H₂O

P7626

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Phenylmethansulfonylfluorid, ≥98.5% (GC)

L4509

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Natriumdodecylsulfat, ReagentPlus^®, ≥98.5% (GC)

G9012

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Glycerin, ≥99.5%

P2714

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Proteasehemmer-Cocktailpulver, for general use, lyophilized powder

E9884

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Ethylendiamintetraessigsäure, ACS reagent, 99.4-100.6%, powder

49781

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Glycerin -Lösung, 83.5-89.5% (T)

E6758

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Ethylendiamintetraessigsäure, anhydrous, crystalline, BioReagent, suitable for cell culture

S6546

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E8008

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Ethylendiamintetraessigsäure -Lösung, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

71725

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Natriumdodecylsulfat, BioUltra, Molecular Biology, ≥99.0% (GC)

G2025

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05030

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59222C

SAFC

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Glycerin, BioUltra, Molecular Biology, anhydrous, ≥99.5% (GC)

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