For use with blood, add the stock solution at a 1:10 dilution, corresponding to 100 µL of reconstituted cocktail per 1 mL of blood, to achieve a 1X final concentration. The optimal concentration may vary depending on the specific application. If there is an anticipated delay in processing whole blood samples, the addition of protease inhibitors may help minimize protein degradation.
Fortfahren mit
P2714
Protease Inhibitor Cocktail
lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder
Synonym(e):
Proteaseinhibitor-Cocktail aus Rinderlunge, Proteaseinhibitor-Mix
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 btl | Warenkorb auf Verfügbarkeit prüfen | € 132,00 |
Über diesen Artikel
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
lyophilized powder
Solubility:
water: soluble
Storage temp.:
−20°C
Technischer Dienst
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Unterstützung erhaltenProduktname
Proteasehemmer-Cocktailpulver, for general use, lyophilized powder
biological source
bovine lung
Quality Segment
form
lyophilized powder
input
general use sample(s)
solubility
water: soluble
storage temp.
−20°C
General description
Dieser Proteaseinhibitor-Cocktail wurde für den allgemeinen Gebrauch optimiert und geprüft. Er besteht aus wasserlöslichen Proteaseinhibitoren, die Serin-, Cystein- und Metalloproteasen wirksam hemmen. Dieses Produkt wird als lyophilisiertes Pulver bereitgestellt.
Application
Proteaseinhibitor-Cocktail-Pulver wird als Komponente verwendet:
- in Puffer für Radioimmunopräzipitationsassays zur Lyse von Kristallinproben der Augenlinse von Vögeln für die Western-Blot-Analyse
- in Phosphatpufferlösung (PBS) zum Mahlen von Blättern, die rekombinante Proteine exprimieren, um virusähnliche Partikel (VLPs) aufzureinigen
- in PBS zur Auflösung einer lyophilisierten Hautsekretprobe von A. loloensis zur Peptidaufreinigung
- in 0,1 M NaCl-Lösung, um Hautsekrete von Fröschen zu waschen und zu sammeln
Biochem/physiol Actions
Die Bestandteile dieser Mischung wie AEBSF und Aprotinin hemmen Serinproteasen, unter anderem Trypsin, Chymotrypsin und Plasmin. Bestatin hemmt Aminopeptidasen. E-64 wirkt gegen Cysteinproteasen. EDTA hemmt Metallproteasen. Leupeptin wirkt sowohl gegen Serin- als auch Cysteinproteasen.
Hemmt Serin-, Cystein- und Metallproteasen.
Preparation Note
Dieses Produkt wird als lyophilisiertes Pulver bereitgestellt. Eine Flasche ermöglicht die Zubereitung von 100 mL einer Cocktaillösung.
Eine Flasche ergibt 100 mL des Cocktails, eine Menge, die zur Hemmung der Proteasen in ca. 20 g Zellmasse ausreicht.
Nicht alle Extrakte enthalten dieselbe Zahl endogener Enzyme und ggf. muss die Menge des benötigten Cocktails angepasst werden.
Nicht alle Extrakte enthalten dieselbe Zahl endogener Enzyme und ggf. muss die Menge des benötigten Cocktails angepasst werden.
Other Notes
Diese Mischung enthält folgende Bestandteile:
2 mM AEBSF
0,3 μM Aprotinin
116 μM Bestatin
14 μM E-64
1 mM EDTA
1 μM Leupeptin
Jede Komponente hat spezifische hemmende Eigenschaften.
2 mM AEBSF
0,3 μM Aprotinin
116 μM Bestatin
14 μM E-64
1 mM EDTA
1 μM Leupeptin
Jede Komponente hat spezifische hemmende Eigenschaften.
Nur für Forschungszwecke. Nicht für den Arzneimittel-, Haushalts- oder sonstigen Gebrauch vorgesehen. Informationen zu den Gefahren und zur sicheren Handhabung sind dem Sicherheitsdatenblatt zu entnehmen.
Disclaimer
Das lyophilisierte Pulver ist bei einer Lagertemperatur von -20 °C 4 Jahre lang haltbar.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description for general use, lyophilized powder | description for use with bacterial cell extracts, lyophilized powder | description for use with mammalian cell and tissue extracts, DMSO solution | description for use in purification of Histidine-tagged proteins |
| form lyophilized powder | form lyophilized powder | form DMSO solution | form tablet |
| biological source bovine lung | biological source - | biological source bovine lung | biological source bovine lung |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| input general use sample(s) | input mammalian sample(s) | input mammalian sample(s) | input general use sample(s) |
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Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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How much of the reconstituted cocktail should I add to 1mL of blood?
1 answer-
Helpful?
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Can I dissolve this product in DMSO (for increased shelf life of the aliquotes at -80degC) rather than water?
1 answer-
This product is water-soluble. The solubility in DMSO is have not been investigated. However, DMSO at concentrations above 1% can destabilize the protein structure.
The product is packaged in a 10 mL serum vial with a crimp cap. Dissolve the contents of the vial in 10 mL of working buffer, then transfer to another container for dilution to 100 mL. Store the solution in working aliquots for at least 1 month -20 C, to avoid repeated freeze/thaw cycles. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/061/p2714dat-ms.pdfThere are several Protease Inhibitor Cocktail formulations that are prepared in DMSO that may be of interest. See the links available below to review these options.
https://www.sigmaaldrich.com/US/en/product/sigma/p1860
https://www.sigmaaldrich.com/US/en/product/sigma/p8215
https://www.sigmaaldrich.com/US/en/product/sigma/p8340Helpful?
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What reagent and volume do I reconstitute this vial?
1 answer-
One bottle prepares 100 mL of cocktail solution. The product is packaged in a 10 mL serum vial with a crimp cap. Dissolve the contents of the vial in 10 mL of water or of any working lysis buffer, then transfer to another container for dilution to 100 mL.
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In un estratto vegatale di 1 mL quanto microL devo aggiungere di cocktail?
1 answer-
Not all extracts contain the same levels of endogenous enzymes, so it is necessary to empirically determine the required volume of cocktail. For reference, one mL of the P2714 cocktail solution is recommended for the inhibition of endogenous enzymes equivalent to 1 mg of USP pancreatin. One bottle is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.
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what is the concentration of P2174 per skin/tissue piece?
1 answer-
We do not measure the concentration of P2714 per skin/tissue piece. Instead, the measured components are: AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. The amount in each vial is 89 mg. This material is very hygroscopic. It is recommended to prepare the entire package rather than attempt to weigh out specific amounts.
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How much PIC do I need to use in salivary samples for Western Blot?
1 answer-
One bottle of the lyophilized powder makes 100 mL of the cocktail. For salivary samples, a 1:500 dilution factor has been known to work well.
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Hi Dose this product P2714, include EDTA? what proteases do it inhibit? Thanks, Camilla
1 answer-
P2714 includes EDTA at 1 mM. The components of this mixture, such as AEBSF and aprotinin, act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin among others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
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Wie löse ich das Pulver in 100 ml aqua dest???? Wie lagere ich das dann?
1 answer-
This product is packaged in a 10 mL serum vial with a crimp cap. Dissolve the contents of the vial in 10 mL of water or working buffer, then transfer to another container for dilution to 100 mL. Store the solution in working aliquots for at least 1 month -20 C, to avoid repeated freeze/thaw cycles. See the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/061/p2714dat-ms.pdfHelpful?
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I intend on taking 1mL samples of blood to give serum and test hormone levels in these samples. Should I add the solubilised protease inhibitor cocktail (p2714) directly to the blood or after centrifugation (which could be a few hours later)?
1 answer-
A short delay may be negligible, however, it would not be detrimental to add protease inhibitors to whole blood samples if an extended delay is anticipated. This would ensure the best results. Please see the link below to review a publication of a similar nature:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817059/Helpful?
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How much powder is in each bottle?
1 answer-
The amount in each vial is 89 mg. This material is very hygroscopic. It is recommended to prepare the entire package rather than attempt to weigh out specific amounts.
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