G3272

Guanidine hydrochloride

Molecular Biology, ≥99%

• Synonym(s): Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
• Linear Formula: NH₂C(=NH)NH₂ · HCl
• CAS Number: 50-01-1
• Molecular Weight: 95.53
• UNSPSC Code: 12352107
• NACRES: NA.31
• PubChem Substance ID: 24895119
• EC Number: 200-002-3
• Beilstein/REAXYS Number: 3591990
• MDL number: MFCD00013026

Properties

• Product Name: Guanidine hydrochloride, Molecular Biology, ≥99%
• InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• SMILES string: Cl[H].NC(N)=N
• biological source: synthetic (organic)
• grade: Molecular Biology
• assay: ≥99%
• form: crystalline powder
• storage condition: (Tightly closed. Dry. )
• technique(s): RNA extraction: suitable
• impurities: ≤0.3% water (Karl Fischer)
• color: white
• pH: (25 °C, 4.6 - 6/573 g/L)
• mp: 180-185 °C (lit.)
• solubility: H₂O: 6 M, clear, colorless
• density: 1.3 g/cm³ (lit.)
• cation traces: Pb: ≤5 ppm
• UV absorption: λ: 260 nm A_max: ≤0.10; λ: 290 nm A_max: ≤0.05
• foreign activity: DNase, RNase, none detected
• Quality Level: 400
• Gene Information: human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)

Description

Application

Guanidine hydrochloride has been used:

• as a component of the extraction buffer for the extraction of proteoglycans[1]
• in extraction during protein fractionation of ATDC5 cell lines[2]
• as a chemical additive to study its effective absorbance spectra in structural analysis[3]
• to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer[4]
• as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study[5]
• in RNA isolation to dissociate nucleoproteins and inhibit RNase

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule.[6][7] It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo.[6] It is a potent denaturant and inactivator of several enzymes and proteins.[7][8] GuHCl can inactivate aminoacylase and papain.[8] It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor.[8] At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).[9]

Disclaimer

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Features and Benefits

• Highly versatile surfactant for your cell biology and biochemical research
• Suitable for sensitive molecular biology applications
• Free from Exonuclease, Dnase and Rnase
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

General description

Guanidine hydrochloride, also known as aminoformamidine hydrochloride, is a versatile compound widely used in molecular biology and biochemical research. Functioning as a potent chaotropic agent, it disrupts protein and nucleic acid structures, making it invaluable for purifying proteins, particularly mRNA. Its denaturing capabilities extend to unfolding proteins, aiding their purification and subsequent refolding, and it can even solubilize denatured insoluble proteins at higher concentrations.

At lower concentrations, guanidine hydrochloride exhibits a unique effect, promoting the refolding of denatured proteins and restoring enzymatic activity. This property is advantageous for protein renaturation studies. In RNA extraction, guanidine hydrochloride acts as a robust denaturant, disrupting cell structures and inactivating RNA enzymes, ensuring the integrity of extracted RNA. In summary, guanidine hydrochloride is a versatile compound with applications in protein purification, nucleic acid isolation, and protein refolding studies, showcasing its multifaceted utility in molecular biology and biochemical research.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 + H332,H315,H319
• pcodes: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves