This product is stored and shipped at ambient temperature. In the powder form, this material is quite robust and does not require controlled temperature storage. This item is stable at room temperature until the lot specific expiration date.
L6876
Lysozyme from chicken egg white
powder or granules, ≥39,000 units/mg protein
Synonyme(s) :
Mucopeptide N-acétylmuramoylhydrolase, Muramidase
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1 G
74,30 €
5 G
256,00 €
10 G
375,00 €
25 G
1 060,00 €
100 G
3 290,00 €
74,30 €
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
MDL number:
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Laissez-nous vous aiderNom du produit
Lysozyme from chicken egg white, powder or granules, ≥39,000 units/mg protein
biological source
chicken egg white
form
powder or granules
specific activity
≥39,000 units/mg protein
mol wt
single-chain 14.3 kDa
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
UniProt accession no.
application(s)
cell analysis
storage temp.
room temp
Quality Level
Gene Information
chicken ... LYZ(396218)
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Cet article | 62970 | L3790 | L4919 |
|---|---|---|---|
| specific activity ≥39,000 units/mg protein | specific activity ~100000 U/mg | specific activity - | specific activity ≥40,000 units/mg protein |
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| biological source chicken egg white | biological source chicken egg white | biological source chicken egg white | biological source chicken egg white |
| Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information - |
| form powder or granules | form powder | form liquid | form lyophilized powder |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) cell analysis |
Application
L′enzyme dégrade la paroi cellulaire des bactéries ; elle est utilisée pour préparer des sphéroplastes.
Biochem/physiol Actions
Le lysozyme hydrolyse les liaisons β(1→4) entre les résidus d′acide N-acétylmuramique et de N-acétyl-D-glucosamine dans le peptidoglycane et entre les résidus de N-acétyl-D-glucosamine dans la chitodextrine. Les cellules à Gram positif sont très susceptibles à cette hydrolyse, car leur paroi cellulaire contient une proportion élevée de peptidoglycanes. Les cellules à Gram négatif sont moins susceptibles, en raison de la présence d′une membrane externe et d′une proportion plus faible de peptidoglycanes. Cependant, ces cellules peuvent être hydrolysées en présence d′EDTA, qui chélate les ions métalliques de la membrane externe des bactéries.
L′enzyme est active dans une vaste gamme de pH (de 6,0 à 9,0). À pH 6,2, une activité maximale est observée sur une plus vaste plage de forces ioniques (de 0,02 à 0,100 M) qu′à pH 9,2 (0,01 à 0,006 M).
L′enzyme est active dans une vaste gamme de pH (de 6,0 à 9,0). À pH 6,2, une activité maximale est observée sur une plus vaste plage de forces ioniques (de 0,02 à 0,100 M) qu′à pH 9,2 (0,01 à 0,006 M).
Le lysozyme hydrolyse les liaisons β(1→4) entre les résidus d′acide N-acétylmuramique et de N-acétyl-D-glucosamine dans le peptidoglycane et entre les résidus de N-acétyl-D-glucosamine dans la chitodextrine. Les cellules à Gram positif sont très susceptibles à cette hydrolyse, car leur paroi cellulaire contient une proportion élevée de peptidoglycanes.
Features and Benefits
La lysozyme de blanc d'œuf de poule est une enzyme polyvalente aux propriétés uniques qui en font un produit efficace dans diverses applications, comme la dégradation de la paroi des cellules bactériennes, l'extraction d'ADN génomique et l'emploi en tant qu'étalon externe dans l'analyse de masse. Elle est fiable, active sur une large plage de pH et disponible en différents conditionnements.
Other Notes
Pour en savoir plus sur les enzymes pour l′analyse des glucides complexes, rendez-vous sur www.sigma-aldrich.com/enzymeexplorer.
Une unité permet de lyser 0,6 μg de Micrococcus lysodeikticus par minute selon une analyse par turbidimétrie à 600 nm sur une suspension tamponnée à pH 6,2 à 25 °C.
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Réf. du produit
Description
Tarif
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocoles
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
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We typically store our lyophilized lysozyme at -20C, but one recent order (shipped at room temperature) was left at room temperature, sealed, for several weeks. Will it still be active?
1 answer-
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I have ordered this product and stored in -70 degree C. But there is no dilution preparation method available here. Can you please tell me hot to prepare that.
1 answer-
This product may be prepared in 10 mM Tris-HCl, pH 8.0 at 10 mg/mL. The material is also soluble in water at 10 mg/mL. Aqueous solutions are stable for at least one month when stored at 2-8 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/938/215/l6876dat-ms.pdfHelpful?
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What's the best buffer and pH for the preparation of lysozyme stock solution, and can I incubate it at room temperature for 1 day?
1 answer-
This product can be resuspended in 350 µL of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl,
1 mM EDTA, and 5% [w/v] TRITON® X-100). The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).During this assay, the end-user should Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Cat. No. T0918) with 25 µg/mL tetracycline (Cat. No. T3383) and 25 µg/mL ampicillin (Cat. No. A9518). The
optimal pH for E. coli cell lysis is 8.0 ± 0.1.16For more information please read through the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/938/215/l6876dat-ms.pdf
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What is the amino acid sequence of Product L6876, Lysozyme from chicken egg white?
1 answer-
Lysozyme is a single chain polypeptide of 129 amino acids. The amino acid sequence of chicken egg white Lysozyme is described in the Journal of Biological Chemistry, 238, 2698-2707 (1963).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product L6876, Lysozyme from chicken egg white, be inactivated by heat?
1 answer-
Solutions of Lysozyme may be inactivated by boiling for 40 seconds at 100°C at an alkaline pH.
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How can Product L6876, Lysozyme from chicken egg white, be reconstituted and can stock solutions be prepared?
1 answer-
For the lysis procedure, use a freshly prepared solution of 500,000 units/ml in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should be stable for at least one month when stored between 2-8 °C.
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Under what conditions can Product L6876, Lysozyme from chicken egg white, be used to lyse E. coli?
1 answer-
The following procedure is for the lysis of E. coli. It may be used as a guideline for other species.1. Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Product No. T 0918) with 25 μg/ml tetracycline (Product No T 3383) and 25 μg/ml ampicillin (Product No. A 9518).2. Centrifuge 1-2 ml samples of the overnight culture.3. Resuspend the pellets in 350 μl of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl, 1 mM EDTA, and 5% [w/v] TRITON® X-100).4. Add 25 μl of a freshly prepared lysozyme solution (500,000 units/ml in 10 mM Tris-HCl, pH 8.0).5. Mix by vortexing for 3 seconds.6. Incubate the lysis mixture for 30 minutes at 37 °C7. After incubation, place the tube containing the lysis mixture in a boiling water bath for exactly 40 seconds.8. Centrifuge the lysis mixture at 14,000 x g.9. Remove the pellet (cell debris) from the tube using a sterile toothpick.10. Plasmid DNA from the supernatant may then be purified and analyzed.
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