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71725

Sigma-Aldrich

Sodium dodecyl sulfate

BioUltra, for molecular biology, ≥99.0% (GC)

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Synonym(s):
Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
Linear Formula:
CH3(CH2)11OSO3Na
CAS Number:
Molecular Weight:
288.38
Beilstein:
3599286
EC Number:
MDL number:
eCl@ss:
39093306
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

description

anionic

product line

BioUltra

Assay

≥99.0% (GC)

form

powder

mol wt

micellar avg mol wt 18,000

aggregation number

62

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

loss

≤3% loss on drying, 20 °C (HV)

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

transition temp

cloud point >100 °C

solubility

H2O: 0.1 M at 20 °C, clear, colorless

density

0.455 g/cm3

anion traces

chloride (Cl-): ≤200 mg/kg
phosphate (PO43-): ≤1 mg/kg

cation traces

Al: ≤5 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.02

HLB

40

suitability

suitable for electrophoresis

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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L6026L3771L4509
Sodium dodecyl sulfate BioUltra, for molecular biology, ≥99.0% (GC)

71725

Sodium dodecyl sulfate

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Sodium dodecyl sulfate BioXtra, ≥99.0% (GC)

L6026

Sodium dodecyl sulfate

Premium Grade
Sodium dodecyl sulfate BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

L3771

Sodium dodecyl sulfate

Essential+ Grade
Sodium dodecyl sulfate ReagentPlus®, ≥98.5% (GC)

L4509

Sodium dodecyl sulfate

-
assay

≥99.0% (GC)

assay

≥99.0% (GC)

assay

≥98.5% (GC)

assay

≥98.5% (GC)

suitability

suitable for electrophoresis

suitability

-

suitability

suitable for electrophoresis

suitability

-

form

powder

form

powder

form

powder

form

powder

aggregation number

62

aggregation number

62

aggregation number

62

aggregation number

62

cation traces

Al: ≤5 mg/kg, Bi: ≤5 mg/kg, Cd: ≤5 mg/kg, Cr: ≤5 mg/kg, Fe: ≤5 mg/kg, Li: ≤5 mg/kg, Mn: ≤5 mg/kg, Ni: ≤5 mg/kg, Sr: ≤5 mg/kg, Ba: ≤5 mg/kg, Co: ≤5 mg/kg, K: ≤200 mg/kg, Mo: ≤5 mg/kg, Zn: ≤5 mg/kg, Ca: ≤10 mg/kg, Mg: ≤5 mg/kg, Cu: ≤5 mg/kg, Pb: ≤5 mg/kg

cation traces

Al: ≤0.0005%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.001%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.02%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

cation traces

heavy metals (as Pb): ≤10 ppm

cation traces

Na: 7.0-9.0%

General description

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is a versatile anionic surfactant with a broad range of applications in molecular biology, biochemistry, and various industries. Its amphiphilic nature, characterized by a hydrophobic hydrocarbon tail and a hydrophilic sulfate head group, makes it an effective detergent capable of solubilizing and denaturing proteins, lipids, and other hydrophobic molecules.

In protein biochemistry, SDS plays a pivotal role in protein extraction and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Its ability to denature proteins by disrupting their secondary and tertiary structures allows for their uniform migration during gel electrophoresis, enabling the separation and analysis of proteins based on their molecular weight. This technique has become an indispensable tool for identifying and characterizing proteins in biological samples.

Beyond its role in protein analysis, SDS also finds utility in nucleic acid extraction and various hybridization techniques. Its detergent properties aid in the lysis of cells and the release of nucleic acids, facilitating their subsequent purification and analysis. Additionally, SDS is employed in hybridization techniques to promote the annealing of complementary nucleic acid strands, enabling the detection and analysis of specific DNA or RNA sequences.

Application

Sodium dodecyl sulfate has been used:
  • as an ionic surfactant in polystyrene (PS) particle suspension for surfactant-based experiments
  • in phosphate-buffered saline as a component of lysis buffer to homogenize myometrium and leiomyoma tissue
  • as a component of lysis buffer to dissolve formazans
  • as a component of high-salt nuclear extraction buffer

Anionic detergent

Biochem/physiol Actions

Sodium dodecyl sulfate (SDS) serves as an important detergent in the structural biology field and protein folding/unfolding studies. The sulfate groups and alkyl chains of SDS can strongly bind to the positively charged and the hydrophobic residues of proteins. SDS induces the formation of α-helices in a protein structure. At high concentrations, SDS stimulates cooperative unfolding in various proteins such as bovine serum albumin (BSA), hen egg-white lysozyme, -lactalbumin, and -lactoglobulin structures.

Features and Benefits

  • Suitable for molecular biology and biochemical research
  • BioUltra grade for your sensitive applications
  • Free from DNase, RNase, NICKase and protease
  • Tested for trace levels of Anions and Cations
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

  • Undergoes hydrolysis at high temperatures, especially in acidic medium

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 2

Flash Point(F)

338.0 °F

Flash Point(C)

170 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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