MABS829

Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2

clone 3E7.2, from mouse

• Synonym(e): Epidermal growth factor receptor, EC: 2.7.10.1, Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 3E7.2, monoclonal
• Speziesreaktivität: human
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_005219
• UniProt-Hinterlegungsnummer: P00533
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: phosphorylation (pTyr1173)
• Angaben zum Gen: human ... EGFR(1956)

Beschreibung

Allgemeine Beschreibung

Epidermal growth factor receptor (UniProt: P00533; also known as EC: 2.7.10.1; Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is activated by binding of its specific ligands and transforms from a monomeric to active homodimeric form. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine residues (Y992, Y1045, Y1068, Y1148 and Y1173) occurs in the C-terminal domain. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding domains. EGFR activation results in at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. These downstream events modulate several cell functions, including migration, adhesion, and proliferation. Endocytosis and inhibition of the activated EGFR by phosphatases constitute key regulatory mechanisms.

~200 kDa observed; 134.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids surrrounding Tyrosine 1173 of human epidermal growth factor receptor.

Anwendung

Detect phospho EGFR using this mouse monoclonal Anti-phospho-EGFR (Tyr1173), clone 3E7.2, Cat. No. MABS829, used in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected phospho-EGFR (Tyr1173) in A431 cells treated with EGF.

Physikalische Form

Format: Purified

Hinweis zur Analyse

Evaluated by Western Blotting in A431 cells treated with EGF.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho-EGFR (Tyr1173) in 10 µg lysate from A431 cells treated with EGF.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Replaces: 05-483

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1