A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Macromolecular bioscience (2015-07-03)

Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi, Gaetano Giammona

ZUSAMMENFASSUNG

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

D2650

Sigma-Aldrich

Dimethylsulfoxid, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

179124

Sigma-Aldrich

Aceton, ACS reagent, ≥99.5%

472301

Sigma-Aldrich

Dimethylsulfoxid, ACS reagent, ≥99.9%

D8418

Sigma-Aldrich

Dimethylsulfoxid, Molecular Biology

151882

Sigma-Aldrich

Deuteriumoxid, 99.9 atom % D

D2438

Sigma-Aldrich

Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

D5879

Sigma-Aldrich

Dimethylsulfoxid, ReagentPlus^®, ≥99.5%

276855

Sigma-Aldrich

Dimethylsulfoxid, anhydrous, ≥99.9%

179973

Sigma-Aldrich

Aceton, Laboratory Reagent, ≥99.5%

319929

Sigma-Aldrich

1,2-Dichlorethan, ACS reagent, ≥99.0%

D4540

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Dimethylsulfoxid, ≥99.5% (GC), suitable for plant cell culture

41640

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Dimethylsulfoxid, puriss. p.a., ACS reagent, ≥99.9% (GC)

130672

Sigma-Aldrich

N-Hydroxysuccinimid, 98%

G8540

Sigma-Aldrich

L-Glutamin, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

450510

Sigma-Aldrich

Deuteriumoxid, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

284505

Sigma-Aldrich

1,2-Dichlorethan, anhydrous, 99.8%

E7750

Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimid -hydrochlorid, commercial grade, powder

32201

Sigma-Aldrich

Aceton, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

178780

Sigma-Aldrich

Tetrabutylammoniumhydroxid -Lösung, 40 wt. % in H₂O

230189

Sigma-Aldrich

Tetrabutylammoniumhydroxid -Lösung, 1.0 M in methanol

471216

Sigma-Aldrich

Diethylamin, ≥99.5%

G3126

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L-Glutamin, ReagentPlus^®, ≥99% (HPLC)

41639

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Dimethylsulfoxid, BioUltra, Molecular Biology, ≥99.5% (GC)

E26266

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Ethylendiamin, ReagentPlus^®, ≥99%

03449

Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimid -hydrochlorid, ≥99.0% (AT)

E6383

Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimid -hydrochlorid, crystalline

G5792

SAFC

L-Glutamin

320110

Sigma-Aldrich

Aceton, ACS reagent, ≥99.5%

D1435

Sigma-Aldrich

Dimethylsulfoxid, meets EP testing specifications, meets USP testing specifications

E1769

Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimid -hydrochlorid, BioXtra