50933

Guanidine hydrochloride

BioUltra, Molecular Biology, ≥99.5% (AT)

• Synonym(s): Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
• Linear Formula: NH₂C(=NH)NH₂ · HCl
• CAS Number: 50-01-1
• Molecular Weight: 95.53
• UNSPSC Code: 12352107
• NACRES: NA.28
• PubChem Substance ID: 57651284
• EC Number: 200-002-3
• Beilstein/REAXYS Number: 3591990
• MDL number: MFCD00013026

Properties

• Product Name: Guanidine hydrochloride, BioUltra, Molecular Biology, ≥99.5% (AT)
• InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• SMILES string: Cl[H].NC(N)=N
• biological source: synthetic
• grade: Molecular Biology
• product line: BioUltra
• assay: ≥99.5% (AT)
• form: powder or crystals
• technique(s): RNA extraction: suitable
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• ign. residue: ≤0.05% (as SO₄)
• loss: ≤0.05% loss on drying, 20 °C (HV)
• color: colorless to white,
• pH: 4.5-6.0 (25 °C, 6 M in H₂O)
• mp: 180-185 °C (lit.)
• solubility: H₂O: 6 M at 20 °C, clear, colorless
• density: 1.3 g/cm³ (lit.)
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤150 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 6 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.02
• storage temp.: room temp
• Quality Level: 200
• Gene Information: human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)

Description

Application

Guanidine hydrochloride (GdnHCl) has been used as a component of the phosphate buffer saline (PBS) for the incubation of Schlemm′s canal (SC) cells.[1] It has also been used as a component of the digestion buffer for the gelation and expansion of human HeLa229 cells.[2] GdnHCl is also used to isolate RNA.[3]

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GdnHCl) is involved in conformational changes and loss of enzyme activity due to inactivation of the enzyme.[4] It is involved in the loss of activity of alkaline phosphatase (ALPase) enzyme in Haliotis diversicolor.[4] GdnHCl functions by hampering the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo.[5] It can also inactivate enzymes like papain and aminocyclase.[6] Higher concentrations of GdnHCl leads to viral inactivation of herpes simplex virus 1 (HSV-1).[7]

General description

Guanidine hydrochloride (GdnHCl) is a well-known protein and enzyme denaturant.[6][4] It is a small molecule and hydroscopic in nature.[6][6]

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 + H332,H315,H319
• pcodes: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves