The Scepter 3.0 sensors are single use only.
PHCC360KIT
Compteur de cellules automatisé portatif Scepter 3.0
Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors
Scepter 3.0 Handheld Automated Cell Counter
Synonyme(s) :
Cell counting instrument
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6 360.00 CHF
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A propos de cet article
Code UNSPSC :
41131501
Nomenclature NACRES :
NC.56
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Laissez-nous vous aiderFabricant/nom de marque
Scepter
Technique(s)
cell counting: suitable
Taille des particules
6-36 μm
Compatibilité
for use with cells sized 4.0–25.0 μm
Conditions d'expédition
ambient
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Cet article | PHCC340KIT | PHCC360500 | PHCC340050 |
|---|---|---|---|
| compatibility for use with cells sized 4.0–25.0 μm | compatibility for use with cells sized 4.0–25.0 μm | compatibility Scepter for use with cells sized 8.0–25.0 μm | compatibility Scepter for use with cells sized 5.0–15.0 μm |
| technique(s) cell counting: suitable | technique(s) cell counting: suitable | technique(s) cell counting: suitable | technique(s) cell counting: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| manufacturer/tradename Scepter | manufacturer/tradename Scepter | manufacturer/tradename Scepter | manufacturer/tradename Scepter |
| particle size 6-36 μm | particle size 4-18 μm | particle size 6-36 μm | particle size 4-18 μm |
Description générale
Le compteur de cellules automatisé portatif Scepter 3.0 permet d'effectuer un comptage cellulaire rapide dans un format facile d'utilisation que l'on tient à la main. Ce système est uniquement destiné à la recherche ; Scepter 3.0 a été testé sur des types de cellules représentatifs des usages actuels. Après avoir préparé une dilution de la culture de cellules à analyser, les instructions à l'écran guident l'utilisateur au travers du processus d'échantillonnage. Moins de 30 secondes après avoir aspiré un échantillon dans sa sonde, le compteur de cellules Scepter affiche à l'écran les résultats suivants :
Le compteur de cellules Scepter 3.0 est compatible avec les sondes pour compteur de cellules Scepter 3.0 40 µM et 60 µM. Ce kit (réf. PHCC360KIT) contient un paquet gratuit de 50 sondes 60 µM.
- Concentration de cellules
- Taille moyenne des cellules
- Volume cellulaire moyen
- Histogramme de la répartition de la taille ou du volume
Le compteur de cellules Scepter 3.0 est compatible avec les sondes pour compteur de cellules Scepter 3.0 40 µM et 60 µM. Ce kit (réf. PHCC360KIT) contient un paquet gratuit de 50 sondes 60 µM.
Autres remarques
- 1 compteur de cellules automatisé portatif Scepter 3.0
- 1 fiole de billes de test
- 1 station de charge
- 50 sondes (60 µM)
Le compteur de cellules Scepter 3.0 n'est pas compatible avec les sondes des générations précédentes ; il n'est compatible qu'avec les sondes pour compteur de cellules Scepter 3.0 figurant dans la section Produits apparentés.
À utiliser avec
Réf. du produit
Description
Tarif
Code de la classe de stockage
10-13 - German Storage Class 10 to 13
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.
Protocoles
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Contenu apparenté
Instruments de laboratoire au fonctionnement intuitif, outils ergonomiques, données accessibles et solutions avant-gardistes pour les laboratoires pharmaceutiques, de recherche et de biotechnologies.
Il contacellule Scepter™ è un apparecchio portatile che consente di realizzare conteggi cellulari accurati e riproducibili direttamente nella cappa di coltura in meno di 30 secondi.
Scepter™ counting is 7 to 10 times faster than hemocytometry-and also faster than other automated counters. Learn more about how this amazing technology works
Intuitiv bedienbare Laborgeräte, ergonomische Tools, leicht zugängliche Daten und zukunftsweisende Lösungen für Forschungs-, Biotech- und Pharmalabors.
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Can I confirm if the sensors are single-use only please?
1 answer-
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What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?
1 answer-
The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.
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Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?
1 answer-
Please see the answers to the following questions below:
1. Is this appropriate for size 15-23 μm cells?
Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.2. Does the Kit include the charger?
This product does include 60μM sensors and a charging station.3. How many 60/40 μm sensors are included
As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.4. Which sensors can be used with this instrument?
This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
See question 1, this product is suitable for the cell size of 15-23 μm.Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.
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Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?
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The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.
Please see the following manual below for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdfHelpful?
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