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Merck

04-119

Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit monoclonal

culture supernatant, from rabbit

Sinonimo/i:

H4K12Ac, Histone H4 (acetyl K12)

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Informazioni su questo articolo

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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Origine biologica

rabbit

Livello qualitativo

100

Forma dell’anticorpo

culture supernatant

Tipo di anticorpo

primary antibodies

Clone

monoclonal

Reattività contro le specie

human, vertebrates

Produttore/marchio commerciale

Chemicon®
Upstate®

tecniche

ChIP: suitable
dot blot: suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

NM_003538.3

N° accesso UniProt

P62805

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

acetylation (Lys12)

Informazioni sul gene

human ... H4C1(8359)

Categorie correlate

Descrizione generale

11kDa

Immunogeno

Peptide corresponding to Histone H4 containing the sequence [GLG-AcK-GGA] on which Lys12 is acetylated.

Applicazioni

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH Coding Region as a positive locus, and a gene desert region as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were resolved probed with anti-acetyl-Histone H4 (Lys12) (1:1,000). Arrow indicates Acetyl-Histone H4 (Lys12).
Arrow indicates Acetyl-Histone H4 (Lys12) (~11 kDa)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology
This Anti-acetyl-Histone H4 (Lys12) Antibody, rabbit is validated for use in WB for the detection of acetyl-Histone H4 (Lys12).

Azioni biochim/fisiol

Recognizes Histone H4 when acetylated on Lys12.
Wide range of cross-reactivity expected based on sequence homology.

Stato fisico

100 μL of rabbit monoclonal IgG cell culture supernatant with 0.1% sodium azide.

Nota sulla preparazione

2 years at -20°C from date of shipment

Risultati analitici

Routinely evaluated by immunoblot.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Fasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus.
Funato, H; Oda, S; Yokofujita, J; Igarashi, H; Kuroda, M
Testing null
Changning Wang et al.
Journal of medicinal chemistry, 57(19), 7999-8009 (2014-09-10)
Epigenetic enzymes are now targeted to treat the underlying gene expression dysregulation that contribute to disease pathogenesis. Histone deacetylases (HDACs) have shown broad potential in treatments against cancer and emerging data supports their targeting in the context of cardiovascular disease
Jerome Jeanblanc et al.
The international journal of neuropsychopharmacology, 18(9) (2015-03-13)
New strategies for the treatment of alcohol dependence are a pressing need, and recent evidence suggests that targeting enzymes involved in epigenetic mechanisms seems to have great potential. Among these mechanisms, alteration of histone acetylation by histone deacetylases is of
Andrew J Kennedy et al.
Cell reports, 16(10), 2666-2685 (2016-08-30)
Human haploinsufficiency of the transcription factor Tcf4 leads to a rare autism spectrum disorder called Pitt-Hopkins syndrome (PTHS), which is associated with severe language impairment and development delay. Here, we demonstrate that Tcf4 haploinsufficient mice have deficits in social interaction
Daniel M Fass et al.
Neuropharmacology, 64, 81-96 (2012-07-10)
Long-term memory formation is known to be critically dependent upon de novo gene expression in the brain. As a consequence, pharmacological enhancement of the transcriptional processes mediating long-term memory formation provides a potential therapeutic strategy for cognitive disorders involving aberrant
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