04-1572-I

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8

culture supernatant, clone 3E8, from rat

• Sinonimo/i: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Livello qualitativo: 100
• Forma dell’anticorpo: culture supernatant
• Tipo di anticorpo: primary antibodies
• Clone: 3E8, monoclonal
• Reattività contro le specie: mouse
• Reattività contro le specie (prevista in base all’omologia): human (immunogen homology)
• tecniche: ChIP: suitable; ELISA: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_000928
• N° accesso UniProt: P24928
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: phosphorylation (pSer5)
• Informazioni sul gene: human ... POLR2B(5431)

Descrizione

Descrizione generale

RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-5′ CTD residue of RPB1.

~220 kDa observed

Immunogeno

Epitope: Phosphorylated Ser5 at the C-terminus domain (CTD).

Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminal domain (CTD).

Applicazioni

Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8 is a highly specific rat monoclonal antibody, that targets RNA Polymerase & has been tested in western blotting & ELISA.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).

ELISA Analysis: A representative lot was used by an independent laboratory to detect RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Azioni biochim/fisiol

This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser5 of the C-terminus domain (CTD).

Stato fisico

Rat monoclonal IgG2aκ with 0.05% sodium azide.

Unpurified

Nota sulla preparazione

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Risultati analitici

Control
Untreated and lambda phosphotase treated NIH/3T3 cell lysates

Evaluated by Western Blot in untreated and lambda phosphotase treated NIH/3T3 cell lysates.

Western Blot Analysis: A 1:2,000 dilution from a representative lot detected RNA polymerase II subunit B1 (phospho-CTD Ser-5) in 10 µg of untreated and lambda phosphotase treated NIH/3T3 cell lysates.

Altre note

Replaces: 04-1572

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1