05-678
Anti-ubiquityl-Histone H2A Antibody, clone E6C5
clone E6C5, Upstate®, from mouse
Sinonimo/i:
H2AUb, Histone H2A (ubiquityl), H2A histone family, member R, histone 1, H2aa, histone H2A, histone cluster 1, H2aa, H2AK119Ub1
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CHF 941.00
CHF 941.00
Informazioni su questo articolo
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Nome del prodotto
Anti-ubiquityl-Histone H2A Antibody, clone E6C5, clone E6C5, Upstate®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
E6C5, monoclonal
species reactivity
amphibian, mouse, human, frog, rat, monkey
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
frog ... H2Ac1(594915)
human ... H2AC1(221613)
mouse ... H2Ac1(319163)
rat ... H2Ac1(24828)
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Questo articolo | 07-371 | 07-745-I | 07-146 |
|---|---|---|---|
| species reactivity amphibian, mouse, human, frog, rat, monkey | species reactivity human, chicken | species reactivity yeast | species reactivity yeast, chicken, human, rat, Saccharomyces cerevisiae, mouse |
| clone E6C5, monoclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| biological source mouse | biological source rabbit | biological source rabbit | biological source rabbit |
| antibody form purified antibody | antibody form purified antibody | antibody form affinity isolated antibody | antibody form serum |
| technique(s) ChIP: suitable, western blot: suitable, immunocytochemistry: suitable | technique(s) western blot: suitable | technique(s) dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, inhibition assay: suitable (peptide), western blot: suitable | technique(s) ChIP: suitable, western blot: suitable |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
Analysis Note
Acid extracts of HeLa cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected ubiquityl-Histone H2A in acid extracts from HeLa and 10T1/2 cells.
Application
This antibody has been reported by an independent laboratory to detect ubiquityl-Histone H2A in cells fixed with either 3% formaldehyde/0.1% Triton X-100 or methanol (Vassilev, A. 1995).
Chromatin Immunoprecipitation:
Reported by an independent laboratory (Wang, H. 2004).
Epigenetics & Nuclear Function
Histones
Biochem/physiol Actions
Disclaimer
General description
Immunogen
Other Notes
Physical form
Preparation Note
Legal Information
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Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenuto correlato
Signaling Product Guide: Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."
Cancer is a complex disease manifestation. At its core, it remains a disease of abnormal cellular proliferation and inappropriate gene expression. In the early days, carcinogenesis was viewed simply as resulting from a collection of genetic mutations that altered the gene expression of key oncogenic genes or tumor suppressor genes leading to uncontrolled growth and disease (Virani, S et al 2012). Today, however, research is showing that carcinogenesis results from the successive accumulation of heritable genetic and epigenetic changes. Moreover, the success in how we predict, treat and overcome cancer will likely involve not only understanding the consequences of direct genetic changes that can cause cancer, but also how the epigenetic and environmental changes cause cancer (Johnson C et al 2015; Waldmann T et al 2013). Epigenetics is the study of heritable gene expression as it relates to changes in DNA structure that are not tied to changes in DNA sequence but, instead, are tied to how the nucleic acid material is read or processed via the myriad of protein-protein, protein-nucleic acid, and nucleic acid-nucleic acid interactions that ultimately manifest themselves into a specific expression phenotype (Ngai SC et al 2012, Johnson C et al 2015). This review will discuss some of the principal aspects of epigenetic research and how they relate to our current understanding of carcinogenesis. Because epigenetics affects phenotype and changes in epigenetics are thought to be key to environmental adaptability and thus may in fact be reversed or manipulated, understanding the integration of experimental and epidemiologic science surrounding cancer and its many manifestations should lead to more effective cancer prognostics as well as treatments (Virani S et al 2012).
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