324865

PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb

liquid, Calbiochem^®

• Sinonimo/i: Anti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: human
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (1:1000))
• Isotipo: IgG
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C
• modifica post-traduzionali bersaglio: phosphorylation (pTyr845)
• Informazioni sul gene: human ... EGFR(1956)

Descrizione

Descrizione generale

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr⁸⁴⁵.

Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr⁸⁴⁵ in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

This PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr⁸⁴⁵).

Immunogeno

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR

Applicazioni

Immunoblotting (1:1000)

Stato fisico

In Dulbecco′s PBS (w/o Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.4.

Nota sulla preparazione

Following initial thaw, aliquot and freeze (-70°C).

Risultati analitici

Positive Control
A431 cells stimulated with EGF

Altre note

Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem.274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.

The immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Toxicity: Standard Handling (A)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable