386040

Anti-Hsp90 Mouse mAb (AC88)

liquid, clone AC88, Calbiochem^®

• Sinonimo/i: Anti-Hsp82, Anti-Hsp85, Anti-Hsp89
• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: AC88, monoclonal
• Stato: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie (prevista in base all’omologia): all
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (1 µg/mL, colorimetric); Immunoprecipitation ; Immunocytochemistry )
• Isotipo: IgG1
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... HSP90AA1(3320)

Descrizione

Descrizione generale

Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.

Recognizes the ~90 kDa Hsp90 protein.

This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.

Immunogeno

Achyla ambisexualis

Hsp90 isolated from water mold, Achyla ambisexualis

Applicazioni

Immunoblotting (1 µg/ml, colorimetric)

Immunoprecipitation (see comments)

Immunocytochemistry (see comments)

Confezionamento

Please refer to vial label for lot-specific concentration.

Stato fisico

In 10 mM PBS, 50% glycerol, pH 7.2.

Nota sulla preparazione

Following initial thaw, aliquot and freeze (-20°C).

Risultati analitici

Positive Control
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells

Altre note

Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.

Harry, J.L. et al. 1990. Development190, 305.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Toxicity: Standard Handling (A)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable