GR01L

Anti-EGFR (Ab-1) Mouse mAb (528)

lyophilized, clone 528, Calbiochem^®

• Sinonimo/i: Anti-Epidermal Growth Factor Receptor
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 528, monoclonal
• Stato: lyophilized
• non contiene: preservative
• Reattività contro le specie: human
• Non deve reagire con: rat, mouse
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• dilution: (Flow Cytometry (5 µg/mL); Frozen Sections (2.5 µg/mL); Immunofluorescence (5 µg/mL); Immunoprecipitation (1 µg/sample); Neutralization Studies ; Paraffin Sections )
• Isotipo: IgG2a
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

Descrizione

Descrizione generale

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.

Recognizes the EGF receptor protein in A431 cells and normal skin.

Immunogeno

partially purified, human EGFR from human A431 cells

Applicazioni

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

Stato fisico

Lyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.

Nota sulla preparazione

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.

Risultati analitici

Positive Control
A-431 cells or skin tissue

Altre note

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Toxicity: Standard Handling (A)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1