The thickness range of IPFL00010 is 110-145 microns.
IPFL00010
Immobilon® -FL PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence
Sinonimo/i:
Western blotting membrane, blotting membrane, transfer membrane
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Informazioni su questo articolo
UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
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Permettici di aiutartiNome del prodotto
Membrana Immobilon®-FL in PVDF, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
Quality Segment
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, electrophoresis transfer: suitable, western blot: suitable
filter L × W
27 cm × 3.75 m
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
General description
La membrana di trasferimento Immobilon®-FL è una membrana microporosa di fluoruro di polivinilidene (PVDF) con pori di dimensione nominale pari a 0,45 μm, progettata per legare le proteine trasferite da una varietà di matrici di gel. La membrana di trasferimento Immobilon®-FL è idrofoba ed è provvista di una struttura dei pori uniforme con un′elevata capacità di legame per le biomolecole. La membrana assicura un blotting ottimale di proteine con peso molecolare superiore a 20.000. La membrana Immobilon®-FL è caratterizzata da un′autofluorescenza molto bassa in un ampio spettro di lunghezze d′onda di eccitazione/emissione. Tale proprietà la rende ideale per applicazioni di blotting che comportino una immunorivelazione basata sulla fluorescenza. La membrana Immobilon®-FL può essere usata anche per rilevamenti chemiluminescenti o cromogeni di routine.
Features and Benefits
Membrana Immobilon-FL:
- le prima membrana da blotting ottimizzata per i metodi di rivelazione in fluorescenza; la fluorescenza di fondo estremamente ridotta migliora la sensibilità con tutti i protocolli di rivelazione in fluorescenza
- compatibile con tutte le comuni sonde fluorescenti, qualunque siano le lunghezze d'onda di eccitazione e di emissione
- ideale per rilevazioni multiplex e in chemifluorescenza
- compatibile con agenti bloccanti e tamponi standard
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
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| description 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes. | description 1 roll, 27 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes. | description 0.45 μm pore size, 7 X 8.4 cm sheets | description 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes. |
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| compatibility for use with Amido black, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with CPTS, for use with All fluorescence dyes, for use with Ponceau-S red, for use with Coomassie brilliant blue | compatibility for use with Amido black, for use with All fluorescence dyes, for use with Coomassie brilliant blue, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with CPTS, for use with Ponceau-S red | compatibility for use with Amido black, for use with Ponceau-S red, for use with CPTS, for use with Coomassie brilliant blue | compatibility for use with CPTS, for use with Amido black, for use with Ponceau-S red, for use with Coomassie brilliant blue |
| detection method fluorometric, chemiluminescent, colorimetric | detection method chemiluminescent, fluorometric, colorimetric | detection method fluorometric, colorimetric, chemiluminescent | detection method colorimetric, chemiluminescent, fluorometric |
| material white filter, PVDF membrane, plain filter | material plain filter, white filter, PVDF membrane | material white filter, PVDF membrane, plain filter | material PVDF membrane, plain filter, white filter |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
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Classe di stoccaggio
11 - Combustible Solids
Che succede?
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
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What is the thickness of this product?
1 answer-
Helpful?
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Is 0.22 µm PVDF membrane available for fluorescent detection in Western blotting?
1 answer-
Immobilon PSQ, with a pore size of 0.2 µm, is suitable for various detection methods including fluorescent, radioactive, chromogenic, and chemifluorescent detection. But when using Immobilon PSQ in Western Blotting, it is important to consider that there may be increased background noise, which might require more stringent wash steps to ensure accurate results.
Helpful?
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Is it possible to use Immobilon FL instead of Immobilon P for western blots?
1 answer-
Yes, Immobilon FL can be used as an alternative to Immobilon P for western blots.
Helpful?
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Active Filters
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Great for western blots
I have been using this product for close to two and half years now. It is a highly reliable PVDF roll for western blotting. I use it to transfer a wide variety of proteins and use HRP conjugated secondary antibodies for imaging bands. The results have always been sharp, reliable and most importantly, reproducible. I shall buy again when we run out.
- A donation to charity was made for this review
- No
Recommends this productYesHelpful?
