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IPFL20200

Immobilon® -FL PVDF Membrane

10 sheets, 20cm x 20 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

Sinonimo/i:

Western blotting membrane, blotting membrane, transfer membrane

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Informazioni su questo articolo

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
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Nome del prodotto

Immobilon®-FL PVDF Membrane, 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Quality Segment

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, electrophoresis transfer: suitable, western blot: suitable

filter L × W

20 cm × 20 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

General description

The Immobilon®-FL transfer membrane has a nominal pore size of 0.45 μm and is made up of polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. This membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biiomolecules. The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. In addition, the Immobilon®-FL membrane can be used for standard chemiluminescent or chromogenic detection. The Immobilon®-FL is recommended for optimal blotting of proteins with molecular weights greater than 20,000.

Application

Immobilon®-FL PVDF Membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL Membrane:
  • Designed for optimal fluorescent immunodetection
  • Extremely low background improves the sensitivity of all fluorescence detection protocols
  • suitable for all commonly used fluorescent probes at all excitation and emission wavelengths
  • compatible with standard blocking agents and buffers
  • Ideal for multiplexing and chemifluorescence detection

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

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1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

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1 roll, 27 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

detection method

colorimetric, fluorometric, chemiluminescent

detection method

chemiluminescent, colorimetric, fluorometric

detection method

fluorometric, chemiluminescent, colorimetric

detection method

chemiluminescent, fluorometric, colorimetric

material

PVDF membrane, plain filter, white filter

material

plain filter, white filter, PVDF membrane

material

white filter, plain filter, PVDF membrane

material

plain filter, white filter, PVDF membrane

compatibility

for use with CPTS, for use with Amido black, for use with Ponceau-S red, for use with Coomassie brilliant blue

compatibility

for use with Amido black, for use with Ponceau-S red, for use with Coomassie brilliant blue, for use with CPTS

compatibility

for use with Amido black, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with CPTS, for use with Coomassie brilliant blue, for use with All fluorescence dyes, for use with Ponceau-S red

compatibility

for use with Amido black, for use with All fluorescence dyes, for use with Coomassie brilliant blue, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with CPTS, for use with Ponceau-S red

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®


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Classe di stoccaggio

11 - Combustible Solids

Che succede?

WGK 3

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable



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