MABE338

Anti-DBF4, clone 7H8.1 Antibody

clone 7H8.1, from mouse

• Sinonimo/i: Protein DBF4 homolog A, Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 7H8.1, monoclonal
• Reattività contro le specie: human
• tecniche: western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_006707
• N° accesso UniProt: Q9UBU7
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... DBF4(10926)

Descrizione

Descrizione generale

DBF4, also known as Protein DBF4 homolog A, Activator of S phase kinase, also known as Chiffon homolog A or DBF4-type zinc finger containing protein 1, and encoded by the gene DBF4/ASK/DBF4A/ZDBF1, is the regulatory subunit for the cell cycle kinase CDC7. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at ′Ser-40′ and ′Ser-53′ and then is involved in regulating the initiation of DNA replication during cell cycle. . DBF4 Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6 proteins. DBF4 is localized to the nucleus. DBF4 is expressed in expressed in many cells particularly high in testis, thymus and most cancer cells.

~77 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogeno

Epitope: Near C-terminus

GST-tagged recombinant protein corresponding to human DBF4 near the C-terminus.

Applicazioni

Anti-DBF4 antibody, clone 7H8.1 detects level of DBF4 & has been published & validated for use in DBF4.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DBF4 in 200 µg of human thymus tissue lysate.

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Nota sulla preparazione

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DBF4 in 200 µg of HepG2 cell lysate.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable