Yes, both MSIPS4W10 and S5EM077I10 correspond to the same plate, identified by the description: MultiScreen HTS IP PVDF 0.45um White Sterile 10pk. MSIPS4W10 is the public catalog number, whereas S5EM077I10 is a custom order request for a specific customer.
MSIPS4W10
Piastra Multiscreen® a 96 pozzetti, membrana in PVDF idrofoba
pore size 0.45 μm, sterile
Multiscreen® 96 well Plate, hydrophobic PVDF membrane
Sinonimo/i:
Piastra a 96 pozzetti idrofoba, Piastra a 96 pozzetti in PVDF
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Informazioni su questo articolo
Codice UNSPSC:
41104923
eCl@ss:
32039006
NACRES:
NB.22
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Servizio Tecnico
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Permettici di aiutartiMateriali
Durapore PVDF membrane
flat bottom wells
white acrylic plate
Livello qualitativo
Descrizione
Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.
Sterilità
sterile
impiego
Automated
Caratteristiche
hydrophobic
lid
Confezionamento
pkg of 10 plates
Produttore/marchio commerciale
MultiScreen®
tecniche
ELISPOT: suitable
cell based assay: suitable
dot blot: suitable
Superficie filtrante
0.28 cm2
Dimens. piastra
96 wells
Volume massimo del pozzetto
300 μL
Volume d’esercizio
50-250 μL
Matrice
Immobilon®-P
Dimensione pori
0.45 μm pore size
Tipo di binding
high binding surface
Compatibilità
centrifuge & manifold
Condizioni di spedizione
ambient
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Questo articolo | MSHVN4B | MAIPN4550 | MSIPN4B10 |
|---|---|---|---|
| binding type high binding surface | binding type low binding surface | binding type high binding surface | binding type high binding surface |
| material PVDF membrane (hydrophobic), flat bottom wells, acrylic | material Barex® , PVDF membrane (hydrophilic), flat bottom wells, titanium dioxide | material PVDF membrane (hydrophobic), acrylic , flat bottom wells | material Barex® , PVDF membrane (hydrophobic), flat bottom wells, titanium dioxide |
| sterility sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® | manufacturer/tradename MultiScreen® |
| working volume 50-250 μL | working volume 50-250 μL | working volume 50-250 μL | working volume 50-250 μL |
Descrizione generale
The polyvinylidene fluoride (PVDF) membrane plates are recommended, over a mixed cellulose ester format, due to slightly improved binding of capture antibody and superior performance in spot detection, particularly for fluorescent applications.
Note legali
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
Protocolli
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Contenuto correlato
This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.
"Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. By virtue of this assay’s unsurpassed sensitivity, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible before are now relatively easy. Recent improvements to the design of multiwell microplates, including use of membranes with reduced background fluorescence, have bolstered the widespread application of Elispot assays. When assay sensitivity, ease of use, and cost are all taken into consideration, the Elispot platform is likely the superior choice for the development of multifunctional T cell assays for the research, therapeutic, and diagnostic communities."
MultiScreen® Filter Plates
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Do MSIPS4W10 and S5EM077I10 refer to the same plate?
1 answer-
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Active Filters
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Great plate for ELISPOTS
These plates give a strong ELISPOT signal with very low background and work well with either a vacuum manifold plate washing apparatus or manual washing.
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