This product has not been tested for labeling bacteria internally, including Staphylococcus aureus. Therefore, no data is available for this application. A literature search is recommended for specific applications.
46950
Fluorescein 5(6)-isothiocyanate
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Fluorescein 5(6)-isothiocyanate
Sinonimo/i:
FITC, Fluorescein isothiocyanate
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50 MG
CHF 45.10
250 MG
CHF 145.00
1 G
CHF 428.00
Informazioni su questo articolo
Formula empirica (notazione di Hill):
C21H11NO5S
Numero CAS:
Peso molecolare:
389.38
Beilstein:
1407295
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.32
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Permettici di aiutartiNome Commerciale
BioReagent
Saggio
≥90% (HPLC)
Stato
powder
PM
389.38 g/mol
Colore
orange to dark orange
yellow to dark yellow
Solubilità
acetone: 0.1 g/10 mL, clear to very slightly hazy
Fluorescenza
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
Compatibilità
suitable for fluorescence
Temperatura di conservazione
−20°C
Stringa SMILE
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
MHMNJMPURVTYEJ-UHFFFAOYSA-N
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Questo articolo | F3651 | F7250 | F4274 |
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| fluorescence λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0 | fluorescence - | fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 492 nm; λem 518 nm (green) |
| assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥97.5% (HPLC) |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| product line BioReagent | product line - | product line - | product line - |
Descrizione generale
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Applicazioni
Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography
Confezionamento
Bottomless glass bottle. Contents are inside inserted fused cone.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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excuse me， can the product of Fluorescein 5(6)-isothiocyanate be used to lable bacteria，such as Staphylococcus aureus？ thanks
1 answer-
Helpful?
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Is it suitable for fluorescence microscopy?
1 answer-
This product is tested for fluorescent labeling of proteins. While not tested specifically for use in fluorescent microscopy, this compound is routinely used in cell-based studies applying this technique.
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