B7542

Maleimide Activated BSA

• Sinonimo/i: Maleimide Activated Bovine Serum Albumin
• Codice UNSPSC: 12352111
• NACRES: NA.32

Proprietà

• Origine biologica: bovine
• Livello qualitativo: 200
• Sterilità: non-sterile
• Stato: lyophilized powder
• Concentrazione: 5—6 mg/mL protein
• tecniche: ELISA: suitable; bioconjugation: suitable
• N° accesso UniProt: P02769
• Temperatura di conservazione: −20°C
• Informazioni sul gene: bovine ... ALB(280717)

Descrizione

Descrizione generale

Maleimide Activated BSA is provided in lyophilized, ready to-use form, eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated BSA contains approximately 5-15 maleimide groups for each BSA molecule.. The conjugation reaction with a cysteine containing peptide is performed at pH 6.6-7.0. The extent of conjugation can then be determined by a colorimetric assay with Ellman′s reagent) to detect unreacted thiol groups in the cysteine-containing peptide.

Applicazioni

Maleimide Activated BSA has been used in peptide conjugation.[1]

Maleimide-activated BSA (mBSA) has reactive maleimide groups on its surface available for conjugation with a cysteine-containing peptide or a thiol-containing hapten. The reaction of the maleimide group and the thiol group proceeds rapidly and selectively under mild coupling conditions (pH 6.5-7.5) to yield a stable, covalently linked peptide-protein conjugate that may be used in immunization protocols or in antibody screening methods.

Stato fisico

Each vial contains 5 mg of Maleimide Activated BSA lyophilized from 10 mM sodium phosphate buffer, pH 6.6, with 0.115 mM NaCl, 1 mM EDTA, and 40 mM sucrose as stabilizer.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable