Please see the links below for the Product Information Sheet which is located in the 'DOCUMENTATION' section under 'More Documents':
https://www.sigmaaldrich.com/product/sigma/c9268#product-documentation
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/422/794/c9268enz.pdf
C9268
Carboxypeptidase A from bovine pancreas
(Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution
Sinonimo/i:
Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
500 units | Per conoscere la disponibilità, visualizza il carrello | CHF 145.00 |
2500 units | Per conoscere la disponibilità, visualizza il carrello | CHF 272.00 |
5000 units | Per conoscere la disponibilità, visualizza il carrello | CHF 549.00 |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
Numero CE:
MDL number:
Specific activity:
≥50 units/mg protein
Servizio Tecnico
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Permettici di aiutartigrade
Proteomics Grade
Quality Segment
form
ready-to-use solution
quality
(Type II-PMSF treated)
specific activity
≥50 units/mg protein
mol wt
~35 kDa
purified by
2× crystallization
impurities
≤0.05 BTEE units/mg protein chymotrypsin, ≤10 BAEE units/mg protein trypsin
storage temp.
2-8°C
Application
The enzyme from Sigma has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.
Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase.
Biochem/physiol Actions
Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.
Preparation Note
Treated with phenylmethylsulfonyl fluoride to eliminate trypsin and chymotrypsin activity. Dialyzed and recrystallized: aqueous suspension with toluene added.
Analysis Note
Protein determined by E1%/278
Other Notes
One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.
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Questo articolo | |||
|---|---|---|---|
| description (Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution | description ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas | description TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein | description (Type II-PMSF treated), ≥20 units/mg protein |
| specific activity ≥50 units/mg protein | specific activity ≥6 units/mL packed gel, 25 °C | specific activity ≥10,000 BAEE units/mg protein | specific activity ≥20 units/mg protein |
| grade Proteomics Grade | grade - | grade Proteomics Grade | grade - |
| form ready-to-use solution | form ammonium sulfate suspension | form essentially salt-free, lyophilized powder | form aqueous suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt ~35 kDa | mol wt ~35,250 | mol wt 23.8 kDa | mol wt ~35 kDa |
| impurities ≤10 BAEE units/mg protein trypsin, ≤0.05 BTEE units/mg protein chymotrypsin | impurities - | impurities - | impurities ≤10 BAEE units/mg protein trypsin, ≤0.05 BTEE units/mg protein chymotrpsin |
Parola di avvertenza
Danger
Codici di pericolo
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
10 - Combustible liquids
Che succede?
WGK 2
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
DPI (dispositivi di protezione individuale)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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