Heat inactivation is required based on the specific application or protocol. It is typically necessary for hybridoma cultures or immunological studies that require the inactivation of complement proteins. However, heat inactivation is not required for all applications.
F2442
Siero fetale bovino
USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
Sinonimo/i:
FBS, FCS, sieri, siero
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
Prezzi e disponibilità al momento non sono disponibili
Informazioni su questo articolo
Vai a
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartiOrigine biologica
bovine fetus
Livello qualitativo
Sterilità
sterile-filtered
Nome Commerciale
FBS Classic
Composizione
Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL
Origine
USA origin
tecniche
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
Impurezze
≤10 EU/mL endotoxin
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
1 of 4
Questo articolo | 12103C | F4135 | F0679 |
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin non-USA origin |
| sterility sterile-filtered | sterility - | sterility sterile-filtered | sterility γ-irradiated, sterile-filtered |
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin |
Descrizione generale
Il siero fetale bovino (FBS) contiene una miscela complessa di biomolecole a basso e alto peso molecolare che possiedono funzioni ottimali per la crescita e per l′inibizione della crescita. L'FBS contiene diverse molecole, quali fattori di crescita, proteine, vitamine, oligoelementi, ormoni ecc. Tali molecole sono importanti per la crescita e il mantenimento delle cellule. È un supplemento comunemente utilizzato per i terreni delle colture cellulari.[1][2] L'FBS è derivato dal sangue di vitello. È privo di fibrina e di fattori della coagulazione. La termo-inattivazione dell′FBS blocca il sistema del complemento.[3] L′FBS derivato dal sangue coagulato è il supplemento indefinito più utilizzato nella coltura di cellule eucariotiche, in particolare di mammifero.[4][5] Viene generalmente utilizzato con i terreni classici sviluppati negli anni '50 e '60. Questi includono molti terreni di uso comune quali: Minimum Essential Medium (MEM),[6] Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM), [7]M199[8] e Roswell Park Memorial Institute medium, RPMI-1640.[6]
Applicazioni
Il siero fetale bovino è stato inoltre usato come componente di:
Azioni biochim/fisiol
FBS è un supplemento molto utilizzato in quanto è in grado di svolgere una vasta gamma di funzioni nelle colture cellulari. L′FBS fornisce sostanze nutritive, oltre a fattori di crescita e adesione, e protegge le cellule dal danno ossidativo e dall′apoptosi mediante meccanismi difficilmente riproducibili nei sistemi con terreni privi di siero (SFM).
Risultati analitici
- Esaminato per la presenza di batteri, virus e micoplasmi
- Sottoposto a tripla filtrazione con membrana da 0,1 micron in condizioni asettiche
- Testato per il contenuto in endotossine ed emoglobina
Altre note
Esaminato per la presenza di batteri, virus, micoplasmi, endotossine e per il contenuto di emoglobina. Sottoposto a tripla filtrazione con membrana da 0,1 micron in condizioni asettiche.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Articoli
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.
High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.
Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.
Protocolli
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
Contenuto correlato
Découvrez notre gamme de sérums de veau fœtal (FBS) et identifiez le FBS adapté à vos besoins et à votre budget. Demander un échantillon de sérum.
Entdecken Sie unser Angebot an fötalem Rinderserum (FBS) und bestimmen Sie das richtige FBS für Ihre Laboranforderungen und Ihr Budget. Serumprobe bestellen
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
-
is heat inactivation necessary?
1 answer-
Helpful?
-
-
How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)
1 answer-
Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.
Here's a protocol for heat inactivation:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.Helpful?
-
-
is this Heat Inactivate FBS?
1 answer-
This product has not been heat inactivated.
Helpful?
-
-
can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?
1 answer-
This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317Helpful?
-
-
Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks
1 answer-
Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/Helpful?
-
-
Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?
1 answer-
We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.
Helpful?
-
-
What is the difference between Fetal Bovine Serum F2442 and 12103C?
1 answer-
Product No. 12103C meets most of the specifications of F2442. Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.
Helpful?
-
-
There is a precipitate in my serum. Is my serum contaminated?
1 answer-
Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.
Helpful?
-
-
What is the cloning assay performed on Fetal Bovine Serum, Product F2442?
1 answer-
The cloning efficiency assay is performed on each lot of FBS to verify that each lot will support clonal growth of myeloma cells and/or fusion products. The assay is performed using 5% and 10% concentrations of the FBS.
Helpful?
-
-
Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?
1 answer-
Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored refrigerated at 2-8°C. If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days. For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance. For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C. For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.
Helpful?
-
Active Filters
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica