When trying to isolate mononuclear cells you can use this product but we offer Histopaque that is a mixture of Ficoll and Hypaque. For humans, we offer H8889, Histopaque®-1077 Hybri-Max™ or 1077-1, Histopaque®-1077. For rodents we offer 1083-1, Histopaque®-1083. For isolation of neutrophils, we offer 1119-1, Histopaque®-1119.
F2637
Polisaccarosio 400
BioXtra, Molecular Biology, lyophilized powder
Sinonimo/i:
Polysucrose 400, Ficoll 400
Autenticati per visualizzare i prezzi riservati alla tua organizzazione & contrattuali
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5 G
CHF 83.00
10 G
CHF 174.00
25 G
CHF 264.00
100 G
CHF 762.00
CHF 83.00
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Permettici di aiutartiOrigine biologica
non-animal source
Grado
Molecular Biology
Nome Commerciale
BioXtra
Stato
lyophilized powder
Attività estranea
DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
Temperatura di conservazione
room temp
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Questo articolo | F8636 | F8016 | F4375 |
|---|---|---|---|
| biological source non-animal source | biological source - | biological source - | biological source - |
| grade Molecular Biology, for molecular biology | grade - | grade - | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form - | form powder, spray-dried |
| storage temp. room temp | storage temp. - | storage temp. - | storage temp. room temp |
| product line BioXtra | product line BioXtra | product line BioReagent | product line - |
Descrizione generale
Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.
Applicazioni
È usato nella separazione di cellule e nell′isolamento di organelli.
Suitable for
- hybridization experiments to reduce non-specific binding of labeled probes to the membrane[1]
- preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
- separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
- producing density gradients to separate subcellular components
- vitrification of cell and tissue samples.[2]
- to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).[3]
Caratteristiche e vantaggi
- free from DNases, RNases, phosphatases and proteases
- preserves functional and morphological integrity of the separated cells
Altre note
Un polimero non ionico sintetico del saccarosio.
Note legali
Ficoll is a registered trademark of Cytiva
Prodotti correlati
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
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Articoli
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Protocolli
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Ficoll®-400 ist ein hochverzweigtes Polymer, das durch Copolymerisation von Saccharose und Epichlorhydrin gebildet wird. Ficoll® 400 ist vollständig nicht-ionisch. Aufgrund der großen Anzahl an Hydroxylgruppen ist Ficoll® 400 sehr hydrophil und extrem wasserlöslich.
Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.
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Can you use Product F2637, Ficoll, to isolate mononuclear cells?
1 answer-
Helpful?
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Do you have a protocols for use of Product F2637, Ficoll, in various applications?
1 answer-
Please check the product information sheet for F4375 which has a detailed protocol.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the difference between Ficoll 400 and Ficoll PM 400?
1 answer-
The difference between Ficoll 400 and Ficoll PM 400 is the PM 400 has stricter specifications on the number of microorganisms/g and the endotoxin level is < 0.125 EU/ml
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Can Product F2637, Ficoll, be used as a component of a DNA loading buffer?
1 answer-
This product can be used in a DNA loading buffer with the following recipe to prepare a 6X stock:0.25% bromophenol blue0.25% xylene cyanol FF15% Ficoll
Helpful?
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Is Product F2637, Ficoll, the same as Ficoll 400?
1 answer-
Yes. It is Ficoll 400. This product is graded molecular biology grade and is free of any nucleases
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