The product is essentially a salt-free preparation and should be reconstituted to a minimum concentration of 1 mg/ml with a saline solution containing 150 mM NaCl.
I4506
IgG
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Sinonimo/i:
IgG umana
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10 MG
CHF 233.00
50 MG
CHF 778.00
100 MG
CHF 1’230.00
500 MG
CHF 1’800.00
Informazioni su questo articolo
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Permettici di aiutartiNome del prodotto
IgG, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
conjugate
unconjugated
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
composition
Protein, ≥90%
technique(s)
ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable
storage temp.
2-8°C
Quality Level
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Categorie correlate
1 of 4
Questo articolo | I5131 | I4381 | 56834 |
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form fibers |
| technique(s) ELISA: suitable, immunoelectrophoresis: suitable, dot immunobinding: suitable, western blot: suitable | technique(s) - | technique(s) ELISA: suitable | technique(s) radioimmunoassay: suitable |
| grade reagent grade | grade reagent grade | grade reagent grade | grade - |
| assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥95% (GE) |
Application
Le IgG da siero umano sono state utilizzate come standard alla concentrazione di 1,274 mg/ml nelle analisi ELISA per determinare le risposte anticorpali a Porphyromonas gingivalis e Prevotella intermedia.[1] Sono state utilizzate per l′immunomarcatura delle cellule Vero, BHK e PtK2 alla concentrazione di 0,2 mg/ml.[2] Le IgG da siero umano sono state utilizzate come standard nell′SDS-PAGE degli anticorpi anti-Shigella.[3]
Biochem/physiol Actions
Gli anticorpi del sottotipo IgG sono le immunoglobuline sieriche più abbondanti del sistema immunitario. Secreti dalle cellule B, si trovano nel sangue e nei fluidi extracellulari e proteggono l′organismo dalle infezioni causate da batteri, funghi e virus. Le IgG materne vengono trasferite al feto attraverso la placenta che è vitale per le difese immunitarie del neonato contro le infezioni.[4]
Disclaimer
Salvo diversa indicazione nel nostro catalogo o altra documentazione aziendale che accompagna i prodotti, i nostri prodotti sono destinati esclusivamente a scopi di ricerca e non devono essere utilizzati per altri scopi, inclusi ma non solo usi commerciali non autorizzati, usi diagnostici in vitro, usi terapeutici ex vivo o in vivo o qualsiasi tipo di consumo o applicazione riguardante l′uomo o gli animali.
General description
Le IgG umane purificate sono isolate da pool di siero umano normale per frazionamento e cromatografia a scambio ionico. Il prodotto è fornito come polvere liofilizzata sostanzialmente priva di sale (meno dell'1% di sodio).
Related product
N° Catalogo
Descrizione
Determinazione del prezzo
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Datasheet
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How is this standard reconstituted?
1 answer-
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What is the expected MW of the product?
1 answer-
The molecular weight of this product is not determined on a lot to lot basis. According to published literature, the molecular weight of Immunoglobulin G (IgG) is approximately 150 - 160 KDa. Historical data available for this product indicates that in SDS-PAGE electrophoresis this material will have multiple bands ranging from 155 kDa - 220 kDa.
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Necesito saber la secuencia de la proteína o el número para buscarla en uniprot
1 answer-
The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.
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What is the isoelectric point of this
1 answer-
This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.
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Is product I4506, because it is derived from human serum, considered to potential biohazard?
1 answer-
As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdfHelpful?
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What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?
1 answer-
After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.
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What is the significance of absorbance at 280 nm (E280 1% = 14)?
1 answer-
The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).
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what is the expected molecular weight under reducing conditions
1 answer-
I4506, Human IgG, is a tetrameric protein consisting of 2 light chains and 2 heavy. When run on a reducing gel, The heavy chain will be detectable at ~50kDa, and the light chain at ~25kda.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Is Product I4506, IgG from human serum, tested for endotoxin?
1 answer-
No. Product No. I4506 is not tested for endotoxin levels.
Helpful?
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