Unfortunately, the half-life of this insulin within the media at 37 °C has not been tested.
I9278
Insulina
sterile-filtered, BioXtra, suitable for cell culture
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
Prezzi e disponibilità al momento non sono disponibili
Informazioni su questo articolo
Vai a
Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutartiSterilità
sterile-filtered
Livello qualitativo
Nome Commerciale
BioXtra
Stato
solution
Concentrazione
9.5-11.5 mg/mL
tecniche
cell culture | mammalian: suitable
Impurezze
≤50 EU/mL endotoxin
pH
7.9-8.5
N° accesso UniProt
Temperatura di conservazione
2-8°C
InChI
PBGKTOXHQIOBKM-FHFVDXKLSA-N
Informazioni sul gene
human ... INS(3630)
1 of 4
Questo articolo | I3536 | 91077C | 11376497001 |
|---|---|---|---|
| biological source Saccharomyces cerevisiae | biological source Saccharomyces cerevisiae | biological source - | biological source - |
| product line BioXtra | product line - | product line - | product line - |
| Quality Level 400 | Quality Level 200 | Quality Level 300 | Quality Level 100 |
| concentration 9.5-11.5 mg/mL | concentration - | concentration - | concentration - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form solution | form lyophilized powder | form dry powder | form lyophilized (clear, colorless solution after reconstitution) |
Descrizione generale
L′insulina è un ormone polipeptidico a doppia catena prodotto dalle cellule β delle isole pancreatiche. Il suo peso molecolare è circa 5800 Da. Le catene α e β sono unite da due legami disolfuro. La catena α contiene un legame disolfuro interno.
Applicazioni
La soluzione di insulina umana è raccomandata per applicazioni di coltura cellulare a concentrazioni tra 0,5 e 1mL per litro di terreno.
Una soluzione di insulina umana è stata usata per test di tolleranza all′insulina in topi e ratti.[1][2] È anche stata usata per la differenziazione adipogenica di colture derivate da cellule staminali embrionali umane (hESCs) e di hMSCs (cellule stromali mesenchimali umane).[3][4]
Una soluzione di insulina umana è stata usata per test di tolleranza all′insulina in topi e ratti.[1][2] È anche stata usata per la differenziazione adipogenica di colture derivate da cellule staminali embrionali umane (hESCs) e di hMSCs (cellule stromali mesenchimali umane).[3][4]
La soluzione di insulina umana è stata utilizzata come supplemento al terreno Eagle modificato da Dulbecco (DMEM) per la coltura di una linea di cellule epiteliali e di una linea cellulare endometriale umana, al terreno essenziale minimo di Eagle per la coltura di una linea di cellule di adenocarcinoma mammario umano e al terreno 199 per la coltura di una linea cellulare ovaria normale.
Azioni biochim/fisiol
L′insulina è coinvolta in vari processi cellulari, dalla promozione della crescita delle cellule, alla regolazione del ciclo cellulare, fino alla promozione dell′assimilazione del glucosio. Viene usato normalmente come supplemento proteico nelle colture di cellule di mammifero prive di siero per contribuire a stimolare la crescita e il mantenimento più adeguati alle cellule mammifere.
Stato fisico
Stabile a 2-8 °C
Altre note
InChI: 1S/C257H383N65O77S6/c1-29-131(23)205(313-193(339)104-259)252(393)317-204(130(21)22)248(389)288-159(75-82-200(349)350)217(358)282-156(71-78-189(263)335)221(362)308-183-116-403-404-117-184-243(384)305-178(111-324)240(381)294-162(88-123(7)8)225(366)295-168(95-140-53-61-146(329)62-54-140)228(369)283-154(69-76-187(261)333)218(359)290-161(87-122(5)6)223(364)285-158(74-81-199(347)348)220(361)302-174(101-190(264)336)235(376)298-170(97-142-57-65-148(331)66-58-142)231(372)309-182(242(383)304-176(255(396)397)103-192(266)338)115-402-401-114-181(214(355)273-107-194(340)278-153(72-79-197(343)344)216(357)281-151(51-42-84-271-257(267)268)212(353)272-108-195(341)279-166(93-138-46-36-32-37-47-138)227(368)297-167(94-139-48-38-33-39-49-139)230(371)299-171(98-143-59-67-149(332)68-60-143)238(379)320-208(135(27)327)254(395)322-85-43-52-186(322)246(387)286-152(50-40-41-83-258)222(363)321-209(136(28)328)256(398)399)311-250(391)203(129(19)20)316-236(377)164(90-125(11)12)292-229(370)169(96-141-55-63-147(330)64-56-141)296-224(365)160(86-121(3)4)289-210(351)133(25)277-215(356)157(73-80-198(345)346)287-247(388)202(128(17)18)315-237(378)165(91-126(13)14)293-233(374)173(100-145-106-270-120-276-145)301-239(380)177(110-323)280-196(342)109-274-213(354)180(113-400-405-118-185(310-244(183)385)245(386)319-207(134(26)326)253(394)306-179(112-325)241(382)318-206(132(24)30-2)251(392)312-184)307-226(367)163(89-124(9)10)291-232(373)172(99-144-105-269-119-275-144)300-219(360)155(70-77-188(262)334)284-234(375)175(102-191(265)337)303-249(390)201(127(15)16)314-211(352)150(260)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-209,323-332H,29-30,40-43,50-52,69-104,107-118,258-260H2,1-28H3,(H2,261,333)(H2,262,334)(H2,263,335)(H2,264,336)(H2,265,337)(H2,266,338)(H,269,275)(H,270,276)(H,272,353)(H,273,355)(H,274,354)(H,277,356)(H,278,340)(H,279,341)(H,280,342)(H,281,357)(H,282,358)(H,283,369)(H,284,375)(H,285,364)(H,286,387)(H,287,388)(H,288,389)(H,289,351)(H,290,359)(H,291,373)(H,292,370)(H,293,374)(H,294,381)(H,295,366)(H,296,365)(H,297,368)(H,298,376)(H,299,371)(H,300,360)(H,301,380)(H,302,361)(H,303,390)(H,304,383)(H,305,384)(H,306,394)(H,307,367)(H,308,362)(H,309,372)(H,310,385)(H,311,391)(H,312,392)(H,313,339)(H,314,352)(H,315,378)(H,316,377)(H,317,393)(H,318,382)(H,319,386)(H,320,379)(H,321,363)(H,343,344)(H,345,346)(H,347,348)(H,349,350)(H,396,397)(H,398,399)(H4,267,268,271)/t131-,132-,133-,134+,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-,209-/m0/s1
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Farinaz Ghodrati et al.
Scientific reports, 8(1), 8654-8654 (2018-06-07)
At times, it can be difficult to discern if a lack of overlap in reported interactions for a protein-of-interest reflects differences in methodology or biology. In such instances, systematic analyses of protein-protein networks across diverse paradigms can provide valuable insights.
E Comisso et al.
Oncogene, 36(30), 4253-4266 (2017-03-21)
OCT4 (Octamer-binding transcription factor 4) is essential for embryonic stem cell self-renewal. Here we show that OCT4 increases the aggressiveness of high-grade serous ovarian cancer (HG-SOC) by inactivating the Retinoblastoma tumor suppressor pathway and enhancing mitotic stability in cancer cells.
Early cardiac changes in a rat model of prediabetes: brain natriuretic peptide overexpression seems to be the best marker.
Nunes S, et al.
Cardiovascular Diabetology, 12, 44-44 (2013)
Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System.
Jones M et al.
Cytotherapy, 15, 1323-1323 (2013)
Hui-Yu Liu et al.
The Journal of biological chemistry, 284(40), 27090-27100 (2009-08-06)
Subjects with type 1 diabetes mellitus (T1DM) eventually develop insulin resistance and other features of T2DM such as cardiovascular disorders. The exact mechanism has been not been completely understood. In this study, we tested the hypothesis that excessive or inappropriate
Protocolli
Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.
Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.
Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.
Contenuto correlato
Explore our protocol for suspension primary hepatocyte cell culture. Discover our primary human hepatocytes and plating and maintenance media formulations.
-
What's the half-life of this insulin within the media at 37C?
1 answer-
Helpful?
-
-
For how long this can be stored @ RT?
1 answer-
This item is stored refrigerated at 2-8°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time.
Refrigerated products that ship at ambient temperature are considered stable up to 2 weeks for transit time.Helpful?
-
-
Can I aliquot the insulin and freeze it? Is it still functional after thawing?
1 answer-
This product is recommended to be stored at 2-8°C. For research applications, freezing insulin solution is a common practice. However, solutions available on the commercial market for therapeutic use are not frozen to maintain maximum activity. No stability studies have been performed on the shelf life of frozen insulin solutions.
Helpful?
-
-
What is the concentration of this solution in (Units/mL) i.e. if my stock is at a concentration of 11.1 mg/mL, what is that in Units/mL?
1 answer-
The specification for the product does not include units of potency.
However, the potency is typically about 28 -29 IU/mg.Helpful?
-
-
What is the source of this insulin and is the solutuion expected to also contain proinsulin?
1 answer-
This product is a biosynthetic product, produced by recombinant expression in yeast (Saccharomyces cerevisiae), with a synthetic gene insert encoding human insulin precursor. The proinsulin content is not among the testing specifications for this product.
Helpful?
-
-
How is this insulin produced? In E.Coli? Yeast?...?
1 answer-
This product is prepared from recombinant human insulin produced in Saccharomyces cerevisiae.
Helpful?
-
-
Is this product suitable for insulin tolerance tests in mice
1 answer-
This item has not been specifically tested for suitability in insulin tolerance. However, the product has been used successfully in a number of published papers. Please see the link below to review one such article:
https://pmc.ncbi.nlm.nih.gov/articles/PMC2785638/Helpful?
-
-
How long does the insulin remain potent for if stored in the fridge?
1 answer-
This product is assigned an expiration date of approximately 2 years from the date of Quality Release, when stored as directed at 2-8°C. The expiration date is lot specific and reported on the product label, as well as the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/i9278#product-documentationHelpful?
-
-
Is this insulin considered of fast or slow action?
1 answer-
This product is intended for research use only and is not a Therapeutic insulin. The research grade insulin products are not defined by the Therapeutic types. There are analytical standards available for each type. Please see the links below to review additional product options for research use:
Insulin Glargine - Slow acting Insulin
https://www.sigmaaldrich.com/search/insulin-glargine?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20glargine&type=productAspart Insulin - Fast acting Insulin
https://www.sigmaaldrich.com/search/insulin-aspart?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20aspart&type=productLispro Insulin - Fast acting Insulin
https://www.sigmaaldrich.com/search/insulin-lispro?focus=products&page=1&perpage=30&sort=relevance&term=insulin%20lispro&type=productHelpful?
-
-
Is this human insulin suitable for use in mouse cell culture?
1 answer-
Yes, this product is suitable for mouse cell culture and has been cell culture tested on the L929 and 3T3 mouse cell lines. Please see the link below to review peer-reviewed articles that may be of interest:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892261/#:~:text=This%20process%20requires%20DRAM%2D1,and%20permitting%20efficient%20mTORC1%20activation.Helpful?
-
Active Filters
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica