Passa al contenuto
Merck

SRP6053

ProBDNF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Sinonimo/i:

Abrineurin, BDNF, Brain-derived neurotrophic factor

Slide 1 of 1
Autenticati per visualizzare i prezzi organizzativi e contrattuali.

Informazioni su questo articolo

Codice UNSPSC:
12352202
NACRES:
NA.32

Documenti fondamentali

Visualizza tutta la documentazione

Vai a

Servizio Tecnico
Hai bisogno di aiuto? Il nostro team di scienziati qualificati è a tua disposizione.
Permettici di aiutarti

Origine biologica

human

Ricombinante

expressed in E. coli

Saggio

≥95% (HPLC)
≥95% (SDS-PAGE)

Stato

lyophilized

PM

~52 kDa

Confezionamento

pkg of 10 μg

N° accesso NCBI

P23560

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... BDNF(627)

Categorie correlate

Confronta articoli simili

Visualizza il confronto per intero

Mostra le differenze

1 of 4

Questo articolo
SRP3014B3795GF301
ProBDNF human recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Sigma-Aldrich

SRP6053

ProBDNF human

BDNF human Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture

Sigma-Aldrich

SRP3014

BDNF human

Brain-derived neurotrophic factor human BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

B3795

Brain-derived neurotrophic factor human

BDNF, proteina ricombinante umana, esente da componenti di origine animale The BDNF protein is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation & survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system.

Sigma-Aldrich

GF301

BDNF, proteina ricombinante umana, esente da componenti di origine animale

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>98% (SDS-PAGE and HPLC)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

biological source

human

biological source

human

biological source

human

biological source

-

mol wt

~52 kDa

mol wt

27.0 kDa

mol wt

~27 kDa

mol wt

-

form

lyophilized

form

lyophilized

form

lyophilized powder

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

Descrizione generale

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors.[1][2] The precursor form of brain-derived neurotrophic factor (pro-BDNF) undergoes post-translational cleavage to form mature BDNF (mBDNF). The gene encoding this protein is localized on human chromosome 11p14.1.[3][4]

Azioni biochim/fisiol

Precursor form of brain-derived neurotrophic factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) (2) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the tropomyosin-related kinase B (TrkB) kinase receptor and promotes the survival, growth and differentiation of neurons.[1] As proneurotrophins and mature neurotrophins elicit opposite biological effects, pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

Stato fisico

Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.

Nota sulla preparazione

Centrifuge the vial prior to opening.
Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.

Altre note

Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
7K014273
7K194273

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Function and evolution in the NGF family and its receptors.
Ebendal T
Journal of Neuroscience Research, 32(4), 461-470 (1992)
The presence of a single-nucleotide polymorphism in the BDNF gene affects the rate of locomotor adaptation after stroke.
Helm EE
Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 234(2), 341-351 (2016)
11p14.1 microdeletions associated with ADHD, autism, developmental delay, and obesity.
Shinawi M
American Journal of Medical Genetics. Part A, 155A(6), 1272-1280 (2011)
Hao-Yun Zhang et al.
Biochimica et biophysica acta, 1843(12), 2967-2975 (2014-09-23)
Iron accumulation is observed in the substantia nigra of patients with Parkinson's disease. However, it is unknown whether neurotrophic factors, brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) participate in the modulation of neuronal iron metabolism. Here
Chih-Yang Lin et al.
Biochemical pharmacology, 91(4), 522-533 (2014-08-26)
Chondrosarcomas are a type of primary malignant bone cancer, with a potent capacity for local invasion and distant metastasis. Brain-derived neurotrophic factor (BDNF) is commonly upregulated during neurogenesis. The aim of the present study was to examine the mechanism involved
Visualizza tutti i documenti correlati

Questions

  1. Hi, Is this recombinant protein functional?

    1 answer

    1. This product is a growth factor analyzed for Appearance, Purity, and Endotoxin level. Kindly review the following citation where this material was used in Primary rat VM neuron culture.

      Zhang HY, Song N, Jiang H, Bi MX, Xie JX. Brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor inhibit ferrous iron influx via divalent metal transporter 1 and iron regulatory protein 1 regulation in ventral mesencephalic neurons. Biochim Biophys Acta. 2014 Dec;1843(12):2967-75. doi: 10.1016/j.bbamcr.2014.09.010. Epub 2014 Sep 16. PMID: 25239763.

      Helpful?

Reviews

No rating value

Active Filters

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica