A6013

Agarosa

Type I, low EEO

• Sinónimos: 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
• Número CAS: 9012-36-6
• EC Number: 232-731-8
• MDL number: MFCD00081294
• UNSPSC Code: 41105317
• PubChem Substance ID: 24891047
• NACRES: NA.25

Propiedades

• biological source: algae (Gelidium & Gracilaria)
• Quality Level: 200
• type: Type I
• form: powder
• technique(s): electrophoresis: suitable
• impurities: ≤10% water
• EEO: 0.09-0.13
• transition temp: gel point 36 °C ±1.5 °C (1.5% gel)
• gel strength: ≥1200 g/cm² (1% gel)
• anion traces: sulfate (SO₄^2-): ≤0.15%
• suitability: suitable for electrophoresis
• SMILES string: O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

Descripción

General description

Agarose is a polymer isolated from red seaweed, from the species Gelidium and Gracilaria, and it is commonly used for the separation of nucleic acids. Type I agarose is often utilized in molecular biology to make agarose gels for electrophoresis, a technique used to separate and analyze DNA or RNA fragments or proteins based on their size. Agarose may also be used in plant cell culture to create hydrogels, which are gel-like materials that provide a three-dimensional matrix. This provides a supportive and biocompatible environment for plant cells, this mimics physiological conditions and conducts experiments in vivo. They are versatile for various applications in plant biology, such as studying cell growth, tissue culture, and analyzing plant responses to different stimuli.

Application

Type 1 Agarose is commonly utilized for molecular biology:

• for gel electrophoresis of DNA and RNA molecules[1][2]
• for agarose overlay in cell culture, to measure antibacterial activity by radial diffusion[3]
• for agarose overlay in cell culture for radio immunodiffusion assays to quantify antigens[4]
• for agarose overlay in plaque assays to determine viral titer
• to prepare agarose hydrogel that encapsulates a viscous media for 3D cell culture[5]
• as an ingredient of human-tissue-like MRI phantoms[6]
• for diagnostic to analyze patient samples for MRSA genome using pulsed-field gel electrophoresis[2]

Features and Benefits

• Low EEO agarose is useful for routine analysis of nucleic acids
• Suitable for electrophoresis: DNase & RNase free
• Suitable for plant cell culture
• Suitable for mammalian cell culture

Analysis Note

A continuación se presenta una lista de las propiedades asociadas a nuestras agarosas:
Contenido de sulfatos - utilizados como un indicador de pureza, ya que los sulfatos constituyen el principal grupo iónico presente.
Fuerza del gel - la fuerza que debe aplicarse a un gel para provocar su fractura.
Punto de gelificación - la temperatura a la cual una disolución acuosa de agarosa forma un gel cuando se enfría. Las disoluciones de agarosa exhiben histéresis en la transición de líquido a gel - es decir, su punto de gelificación no es el mismo que su temperatura de fusión.
Electroendosmosis (EEO) - movimiento de líquido a través del gel. En un gel de agarosa, los grupos aniónicos están pegados a la matriz y no pueden moverse, pero los contra-cationes disociables pueden migrar en la matriz hacia el cátodo, dando lugar a EEO. Dado que el movimiento electroforético de los biopolímeros suele ser en dirección al ánodo, la EEO puede alterar las separaciones debido a la convección interna.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)