The lipid content of this material has not been determined. Please see the link below to review the current testing performed on this product:
https://www.sigmaaldrich.com/US/en/product/sial/a7906
Please see the link below to review an albumin application guide that may be helpful:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/538/albumin-application-selection-guide.pdf
A7906
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Synonyma:
Albumin bovine serum, BSA, Bovine albumin
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| Vám/SKU | Dostupnost | Cena |
|---|---|---|
50 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 9 270,00 Kč |
100 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 14 300,00 Kč |
500 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 47 100,00 Kč |
1 kg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 47 500,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
blood typing: suitable, microbiological culture: suitable
Impurities:
≤5.0% Loss on drying
9 270,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohlibiological source
bovine
Quality Segment
assay
≥98%
form
lyophilized powder
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
blood typing: suitable, microbiological culture: suitable
impurities
≤5.0% Loss on drying
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT virus, none detected, VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Application
Bovine serum albumin has been used-
- as a component of PCR (polymerase chain reaction) mix
- in the preparation of dissociated cortical neurons
- during immunofluorescent labeling
- as a component of Ca2+-free saline (containing collagenase, hyaluronidase and DNAse) used for bovine tissue digestion
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Prepared using heat shock fractionation.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
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Tato položka | |||
|---|---|---|---|
| description heat shock fraction, pH 7, ≥98% | description heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98% | description lyophilized powder, essentially globulin free, BioReagent, suitable for cell culture | description heat shock fraction, protease free, essentially globulin free, pH 7, ≥98% |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| assay ≥98% | assay ≥98% | assay ≥99% | assay ≥98% |
| technique(s) blood typing: suitable, microbiological culture: suitable | technique(s) electrophoresis: suitable, ELISA: suitable, western blot: suitable | technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, western blot: suitable |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Skladovací třída
11 - Combustible Solids
Co se děje?
WGK 1
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
PPE (osobní ochranné prostředky)
Eyeshields, Gloves, type N95 (US)
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Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
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Are the lipid components of bovine serum albumin (A7906) quantified or were they measured before?
1 answer-
Helpful?
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Can I use this BSA (A7906) to dilute cytokines for use in cell cultures? What is the recommended concentration range for this solution?
1 answer-
This product has not been validated for cell culture use on batch release. It has been primarily cited for immunoassay applications, with a few references to cell culture applications. Please see the links below to BSA products tested for suitability in cell culture:
Suitable for cell culture, low endotoxin
https://www.sigmaaldrich.com/product/sigma/a4919Suitable for cell culture, IgG-free, sterile powder
https://www.sigmaaldrich.com/product/sigma/a3156Suitable for cell culture, 35% sterile solution in DPBS
https://www.sigmaaldrich.com/product/sigma/a7979Helpful?
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Need the exact molecular weight and elemental formula or amino acid sequence for this product.
1 answer-
Bovine Serum Albumin (BSA) has an approximate molecular weight of 66 kDa. Please see the link below to review the BSA sequence information published at UniProt:
https://www.uniprot.org/uniprotkb/P02769/entry#sequencesAdditional information is available in the product datasheet provided at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/340/237/a7906pis.pdfHelpful?
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How long is BSA (A7906) viable at room temperature? 200g was left on benchtop overnight (Approximately 22 hours), will it still work?
1 answer-
This item is stored refrigerated at 2-8°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time.
Refrigerated products that ship at ambient temperature are considered stable up to 2 weeks for transit time.Helpful?
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Is the product A7906, Albumin from bovine serum (BSA), fatty acid-free?
1 answer-
This product is not considered to be fatty acid-free. Please see the link below to review available fatty acid-free product options:
https://www.sigmaaldrich.com/search/bovine-serum-albumin-fatty-acid-free?facet=facet_biological_source%3Abovine&focus=products&page=1&perpage=30&sort=relevance&term=bovine%20serum%20albumin%20fatty%20acid%20free&type=productHelpful?
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After reconstitution in PBS, for how long is the protein stable in solution if stored at 4°C or -20*C?
1 answer-
Prepared BSA solution products also available for purchase. These products have a shelf life ranging from 18 months to 2 years. Many are assigned a restest schedule with no firm expiration date. These products are prepared in saline, DPBS, or Tris. These solutions are filtered products and offered in a range of concentrations. The storage recommendation for these products is 2 - 8 deg. C. In addition, BSA is often used as a carrier protein for less stable compounds that are stored frozen. While there is no established shelf life for a BSA solution stored frozen, it is quite stable. See the link below for a sample Certificate of Analysis for one of the BSA solutions.
Helpful?
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Does this BSA have bovine immunoglobulins?
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This product does contain bovine immunoglobulins. BSA products that do not contain immunoglobulins will state "globulin-free" or "essentially globulin-free" on the product detail page. Product A3156 is an example of an immunoglobulin free BSA product.
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Can Product A7906, Albumin from bovine serum (BSA), be used in immunohistochemistry?
1 answer-
We have not ourselves tested A7906 for use in immunohistochemistry. However, a literature search can turn up publications that cite this use, such as this paper: Surendran, K., et al., Wnt-Dependent Beta-Catenin Signaling Is Activated after Unilateral Ureteral Obstruction, and Recombinant Secreted Frizzled-Related Protein 4 Alters the Progression of Renal Fibrosis. J. Am. Soc. Nephrol., Vol. 16, pp. 2373-2384 (2005).
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Is Product A7906, Albumin from bovine serum (BSA), protease-free?
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We have not tested A7906 in quality control to be protease-free. Other BSA products to suggest that are protease-free would be A3059 or B6917.
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