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Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA
Price: 893 USD
Availability: InStock

heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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Size/SKU	Availability	Price
25 g	Check Cart for Availability	$893.00
100 g	Check Cart for Availability	$2,870.00

About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥98%

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Impurities:

protease, none detected, ≤1.0 EU/mg endotoxin

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Properties

biological source

bovine

Quality Segment

200

product line

BioReagent

assay

≥98%

form

lyophilized powder

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

protease, none detected, ≤1.0 EU/mg endotoxin

loss

≤5%

pH

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT virus, none detected, Protease by FITC, none detected, VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Bovine serum albumin (BSA), a 66 kDa protein, belongs to the family of serum albumins. It is an α-helical, non-glycosylated and globular protein, that has 17-disulfide bonds.

Application

Bovine Serum Albumin has been used:

as a component of cell culture media in cochlear explant culture
organotypic cultures and embryo culture
as a component of phosphate-buffered saline (PBS) in immunostaining of human induced pluripotent stem cell (hiPSC)

Select the right albumin for your application with the Albumin Selection Guide.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Bovine Serum Albumin (BSA) is the most vital constituent of the cell culture media, which aids embryonic stem cells (hESC) differentiation. It acts as a blocking agent in enzyme linked immunosorbent assay (ELISA) to inhibit non-specific binding of antigens and antibodies to the microtiter plates.

Features and Benefits

Tested for use in cell culture
Protease-free
Low endotoxin

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

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This Item	A9085	A2934	A0281
$Bovine Serum Albumin heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%$ Sigma-Aldrich A4919 Bovine Serum Albumin	Sigma-Aldrich A9085 Bovine Serum Albumin	Sigma-Aldrich A2934 Bovine Serum Albumin	Sigma-Aldrich A0281 Bovine Serum Albumin
description heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%	description lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis)	description lyophilized powder, essentially globulin free, low endotoxin, ≥98% (agarose gel electrophoresis)	description lyophilized powder, essentially fatty acid free and essentially globulin free, ≥99% (agarose gel electrophoresis)
assay ≥98%	assay ≥96% (agarose gel electrophoresis)	assay ≥98% (agarose gel electrophoresis)	assay ≥99% (agarose gel electrophoresis)
biological source bovine	biological source bovine	biological source bovine	biological source bovine
origin USA origin	origin USA origin	origin USA origin	origin USA origin
technique(s) cell culture \| mammalian: suitable	technique(s) flow cytometry: suitable, immunofluorescence: suitable	technique(s) ELISA: suitable	technique(s) enzyme immunoassay: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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