This product is not tested for biotin levels or suitability in biotin/streptavidin-mediated detection systems.
A7979
Bovine Serum Albumin solution
35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture
Synonym(s):
Albumin solution from bovine serum, BSA solution
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| Size/SKU | Availability | Price |
|---|---|---|
50 mL | Please contact Customer Service for Availability | $466.00 |
12 x 50 mL | Please contact Customer Service for Availability | $4,300.00 |
About This Item
Biological source:
bovine
Assay:
33-37% total protein basis (UV)
Form:
liquid
Technique(s):
blocking: suitable, cell culture | mammalian: suitable
Impurities:
≤1000 EU/mL Endotoxin level
Concentration:
35% in DPBS
$466.00
Please contact Customer Service for Availability
biological source
bovine
Quality Segment
description
endotoxin tested
sterility
sterile-filtered
product line
BioXtra
assay
33-37% total protein basis (UV)
form
liquid
origin
USA origin
concentration
35% in DPBS
technique(s)
blocking: suitable, cell culture | mammalian: suitable
impurities
≤1000 EU/mL Endotoxin level
pH
6.5-7.5
foreign activity
Mycoplasma Test by Barile On raw material, none detected
shipped in
ambient
storage temp.
2-8°C
General description
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Application
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
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This Item | |||
|---|---|---|---|
| technique(s) blocking: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable | technique(s) cell culture | mammalian: suitable |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| assay 33-37% total protein basis (UV) | assay 28-32% total protein basis (UV) | assay 28-32% protein basis (biuret) | assay ≥99% (agarose gel electrophoresis) |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| Quality Level 400 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form solution | form lyophilized powder |
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Is it suitable for biotin/streptavidin-mediated detection systems?
1 answer-
Helpful?
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IS IT FREE FROM CALCIUM AND MAGNESIUM?
1 answer-
This product is prepared in Dulbeccos Phosphate Buffered Saline without calcium and magnesium.
Helpful?
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What is the concentration of this product?
1 answer-
As shown in the DESCRIPTION section of the Product Detail Page, the BSA concentration for Product A7979, is 35% w/v in DPBS.
Helpful?
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Hi, We have used Bovine serum albumin (A6003) and prepared a 10% BSA solution in buffer. There are also several BSA solutions available, but I can't see any information what BSA solid that was used (Product/Cat number). Has any the same origin?
1 answer-
Product A6003 is a fatty acid-free BSA powder.
See the links below to review the fatty acid-free BSA solutions currently prepared from this material.
A1595, 10% BSA Solution, Fatty acid-free, in DPBS
https://www.sigmaaldrich.com/product/sigma/a1595A9205, 30% BSA Solution, Fatty acid-free, in DPBS
https://www.sigmaaldrich.com/product/sigma/a9205Helpful?
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