This product is not tested for biotin levels or suitability in biotin/streptavidin-mediated detection systems.
A7979
Bovine Serum Albumin solution
35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture
Synonym(s):
Albumin solution from bovine serum, BSA solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 mL | Check Cart for Availability | $411.00 | |
| 12 x 50 mL | Check Cart for Availability | $3,990.00 |
About This Item
Biological source:
bovine
Assay:
33-37% total protein basis (UV)
Form:
liquid
Technique(s):
blocking: suitable, cell culture | mammalian: suitable
Impurities:
≤1000 EU/mL Endotoxin level
Concentration:
35% in DPBS
$411.00
Check Cart for Availability
biological source
bovine
Quality Level
description
endotoxin tested
sterility
sterile-filtered
product line
BioXtra
assay
33-37% total protein basis (UV)
form
liquid
origin
USA origin
concentration
35% in DPBS
technique(s)
blocking: suitable, cell culture | mammalian: suitable
impurities
≤1000 EU/mL Endotoxin level
pH
6.5-7.5
foreign activity
Mycoplasma Test by Barile On raw material, none detected
shipped in
ambient
storage temp.
2-8°C
General description
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Application
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
1 of 1
This Item | |||
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| assay 33-37% total protein basis (UV) | assay 7.0-8.0% protein basis (UV) | assay 28-32% (biuret) | assay 90-120 mg/mL protein basis (biuret) |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) blocking: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) blocking: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 400 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| form liquid | form liquid | form solution | form solution |
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Articles
Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.
Katharina Janssen et al.
Methods in molecular biology (Clifton, N.J.), 1994, 31-39 (2019-05-28)
To better understand and model neurological, in particular neurodegenerative diseases, human induced pluripotent stem cells (hiPSCs) offer a great source for generation of neural cells. We provide a protocol for the differentiation of hiPSc-derived astrocytes in vitro. This protocol not
Mohammed Samer Shaban et al.
Nature communications, 12(1), 5536-5536 (2021-09-22)
Coronaviruses (CoVs) are important human pathogens for which no specific treatment is available. Here, we provide evidence that pharmacological reprogramming of ER stress pathways can be exploited to suppress CoV replication. The ER stress inducer thapsigargin efficiently inhibits coronavirus (HCoV-229E
Brett S Peterson et al.
Cell reports, 24(1), 209-223 (2018-07-05)
SIRT3 is a nicotinamide adenine dinucleotide (NAD+)-dependent mitochondrial protein deacetylase purported to influence metabolism through post-translational modification of metabolic enzymes. Fuel-stimulated insulin secretion, which involves mitochondrial metabolism, could be susceptible to SIRT3-mediated effects. We used CRISPR/Cas9 technology to manipulate SIRT3 expression in
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A7979-12X50ML | 04061833390856 |
| A7979-50ML | 04061833390863 |
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Is it suitable for biotin/streptavidin-mediated detection systems?
1 answer-
Helpful?
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IS IT FREE FROM CALCIUM AND MAGNESIUM?
1 answer-
This product is prepared in Dulbeccos Phosphate Buffered Saline without calcium and magnesium.
Helpful?
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What is the concentration of this product?
1 answer-
As shown in the DESCRIPTION section of the Product Detail Page, the BSA concentration for Product A7979, is 35% w/v in DPBS.
Helpful?
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Hi, We have used Bovine serum albumin (A6003) and prepared a 10% BSA solution in buffer. There are also several BSA solutions available, but I can't see any information what BSA solid that was used (Product/Cat number). Has any the same origin?
1 answer-
Product A6003 is a fatty acid-free BSA powder.
See the links below to review the fatty acid-free BSA solutions currently prepared from this material.
A1595, 10% BSA Solution, Fatty acid-free, in DPBS
https://www.sigmaaldrich.com/product/sigma/a1595A9205, 30% BSA Solution, Fatty acid-free, in DPBS
https://www.sigmaaldrich.com/product/sigma/a9205Helpful?
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