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H4272

Hyaluronidase from bovine testes

Name: Hyaluronidase from bovine testes
Brand: SIGMA
Price: 6950 CZK

Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

Synonyma:

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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Vám/SKU	Dostupnost	Cena
30 mg	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	6 950,00 Kč

O této položce

Číslo CAS:

37326-33-3

UNSPSC Code:

12352204

NACRES:

NA.75

EC Number:

253-464-3

MDL number:

MFCD00131351

Číslo EC:

3.2.1.35 (BRENDA, IUBMB)

Specific activity:

750-3000 units/mg solid

6 950,00 Kč

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Technický servis

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Vlastnosti

sterility

aseptically processed

Quality Segment

200

type

Type IV-S

form

powder

specific activity

750-3000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

composition

Protein, 80-105% biuret

technique(s)

cell culture | embryo: suitable

storage temp.

−20°C

Popis

Application

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass in the isolation of mouse embryos or from newly ovulated eggs, when preparing the eggs for in vitro culture.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Preparation Note

Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

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Tato položka	H3506	H3884	H3631
Sigma-Aldrich H4272 Hyaluronidase from bovine testes	Sigma-Aldrich H3506 Hyaluronidase from bovine testes	Sigma-Aldrich H3884 Hyaluronidase from bovine testes	Sigma-Aldrich H3631 Hyaluronidase from bovine testes
description Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid	description Type I-S, lyophilized powder, 400-1000 units/mg solid	description Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid	description Type VI-S, lyophilized powder, 3,000-15,000 units/mg solid
technique(s) cell culture \| embryo: suitable	technique(s) single cell analysis: suitable, cell culture \| mammalian: suitable	technique(s) -	technique(s) -
specific activity 750-3000 units/mg solid	specific activity 400-1000 units/mg solid	specific activity 750-3000 units/mg solid	specific activity 3,000-15,000 units/mg solid
form powder	form lyophilized powder	form lyophilized powder (essentially salt-free)	form lyophilized powder
mol wt ~55 kDa (four subunits of 14 kDa each)	mol wt ~55 kDa (four subunits of 14 kDa each)	mol wt ~55 kDa (four subunits of 14 kDa each)	mol wt ~55 kDa (four subunits of 14 kDa each)
sterility aseptically processed	sterility -	sterility -	sterility -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Piktogramy

GHS08

Výstražné slovo

Danger

Kódy nebezpečí

H334

Bezpečnostní pokyny

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Skladovací třída

11 - Combustible Solids

Co se děje?

WGK 3

Bod vzplanutí (°F)

Not applicable

Bod vzplanutí (°C)

Not applicable

PPE (osobní ochranné prostředky)

Eyeshields, Gloves, type N95 (US)

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

3 years ago

What's the conversion between Sigma Unit and International Unit for this enzyme ?

1 answer
1. Technical Support
  
  3 years ago
  
  The International Unit, as described by WHO, (1957-01-01), J H Humphrey, is based on, and compared to, the activity of 0.1 mg of the International Standard Preparation'. The value is reported as Turbidity Reducing Units. This product is not tested against an international standard. The activity is measured by the change in absorbance values over time in the presence of hyaluronic acid. See the links below for additional information.
  International Standard for Hyaluronidase reference:
  https://www.sigmaaldrich.com/tech-docs/paper/1257671
  Enzymatic Assay Method applied to this product:
  https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase
  Product Datasheet for H3883, the source product for H4272 - note the Procedure for synovial fluid clarification, page 1:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdf
  
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