A6014

Acridinorange Hemi-(zinkchlorid) (Salz)

For nucleic acid staining in cells or gels

• Synonym(e): 3,6-Bis-(dimethylamino)-acridin-hydrochlorid Zinkchlorid Doppelsalz, 3,6-Bis-(dimethylamino)-acridinium-chlorid Hemi-(zinkchlorid) (Salz), Basic Orange 14
• Lineare Formel: C₁₇H₂₀ClN₃ · HCl · 1/2ZnCl₂
• CAS-Nummer: 10127-02-3
• Molekulargewicht: 369.96
• EC Number: 233-353-6
• UNSPSC Code: 12171500
• NACRES: NA.52
• PubChem Substance ID: 24891048
• MDL number: MFCD00081043
• Colour Index Number: 46005
• Beilstein/REAXYS Number: 3734978

Eigenschaften

• Produktname: Acridinorange Hemi-(zinkchlorid) (Salz), For nucleic acid staining in cells or gels
• InChI key: RAHGLSRJKRXOSY-UHFFFAOYSA-L
• InChI: 1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2
• SMILES string: Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C
• product line: BioReagent
• form: powder
• composition: Dye content, ~80%
• technique(s): nucleic acid detection: suitable
• solubility: ethanol: 2 mg/mL; 4 mg/mL (2-methoxyethanol (EGME)); water: 6 mg/mL (Forms a clear, dark orange or amber solution at 1mg/mL.)
• suitability: suitable for flow cytometry; suitable for microscopy
• storage temp.: room temp
• Quality Level: 200

Beschreibung

Application

Acridinorange, ein zelldurchdringender metachromatischer fluoreszierender kationischer Farbstoff, der DNA und RNA interkaliert, wird in der Fluoreszenz- und Epifluoreszenzmikroskopie verwendet. Der Acridinorange-Farbstoff wurde verwendet, um den Mitochondrien- und Lysosomen-Gehalt mittels Durchflusszytometrie zu analysieren, um Multidrug-Resistenz zu charakterisieren und um Veränderungen in der mitochondrialen Masse während der Apoptose in Ratten-Thymozyten zu messen.

Suitable for

• detection of nucleic acids separated by gel electrophoresis
• fluorescence and epifluorescence microscopy
• analysis of mitochondria and lysosomes by flow cytometry
• DNA staining in apoptosis studies

Biochem/physiol Actions

Acridine orange intercalates into the nucleic acids of double helix and is detectable as green fluorescence at 530 nm. It binds electrostatically to the phosphate groups in single stranded nucleic acids and is detectable at red fluorescence at 640 nm.[1]

Features and Benefits

• 120 microM of acridine orange detects 25-50 ng of purified DNA per band in gels
• differential staining of single- and double-stranded polynucleotides

General description

Acridine orange is a metachromatic fluorescent cationic dye that permeates the cell membrane and intercalates DNA and RNA. It allows for visual detection of nucleic acids on agarose and polyacrylamide gels.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Warning
• hcodes: H341
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)