The expiration date of Accuspin tubes is stated on the box, andits not possible to specify a shorter shelf life after opening. Individual Accuspin tube is sterile until the cap is removed, and the opened box or tube stability can only be determined by the user.
A6929
ACCUSPIN™ System-Histopaque®-1077
sterile-filtered, density: 1.077 g/mL
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Unterstützung erhaltenQualitätsniveau
Sterilität
aseptically filled
Form
solution
Haltbarkeit
Expiry date on the label.
Verpackung
pkg of packaging 40 tubes
Methode(n)
cell culture | mammalian: suitable
Dichte
1.077 g/mL
Anwendung(en)
hematology
histology
Lagertemp.
2-8°C
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Verwandte Kategorien
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Dieser Artikel | A7054 | 10771 | A0561 |
|---|---|---|---|
| form solution | form solution | form liquid | form solution |
| sterility aseptically filled | sterility aseptically filled | sterility sterile-filtered | sterility aseptically filled |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| density 1.077 g/mL | density 1.077 g/mL | density 1.077 | density 1.077 g/mL |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| packaging pkg of packaging 40 tubes | packaging pkg of package 12 tubes | packaging - | packaging package of 100 tubes |
Allgemeine Beschreibung
ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 12mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). Each tube is aseptically filled with 3 mL of Histopaque 1077. Each tube will separate 3-6 mL of anticoagulated blood.
Anwendung
ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.
ACCUSPIN System-Histopaque-1077 is used for the separation of lymphocytes and other mononuclear cells from whole blood and bone marrow using density gradient separation media. Histopaque-1077 has been used for:
- human lymphocyte antigen (HLA) typing
- initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes.
- preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
- isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)citation
- isolation of human lymphocytescitation
- isolation of breast cancer circulating tumor cells (CTCs) from the whole bloodcitation
Biochem./physiol. Wirkung
The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, and resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.
Leistungsmerkmale und Vorteile
ACCUSPIN™ System-Histopaque®-1077 offers numerous advantages, such as:
- Optimal recovery of viable cells
- Permits selective separation of blood cell types
- Prevents cell distortion
- Preserves cell viability
- Minimal extraneous cell interference
- Reproducible performance from batch to batch
- Histopaque is sterile-filtered and endotoxin-tested
- Stable at 2-8°C for at least 2 years when protected from light
Angaben zur Herstellung
Package contains 40 prefilled tubes
Rechtliche Hinweise
Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Resp. Sens. 1 - Skin Sens. 1
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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What is the open box shelf life for Accuspin tubes after opening the box?
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