DAPI is suitable for staining bacterial chromosomes, including those of E. coli.
D9542
DAPI
for nucleic acid staining
DAPI
Synonym(e):
4′,6-Diamidin-2-phenylindol -dihydrochlorid, 2-(4-Amidinophenyl)-6-indolcarbamidin -dihydrochlorid, DAPI -dihydrochlorid
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1 MG
16,40 €
5 MG
69,20 €
10 MG
117,00 €
50 MG
472,00 €
Über diesen Artikel
Empirische Formel (Hill-System):
C16H15N5 · 2HCl
CAS-Nummer:
Molekulargewicht:
350.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
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Unterstützung erhaltenProduktname
DAPI, for nucleic acid staining
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
grade
Molecular Biology
assay
≥98% (HPLC)
form
powder
technique(s)
transfection: suitable
solubility
H2O: 20 mg/mL
PBS: insoluble
ε (extinction coefficient)
30 at 263 nm in H2O at 1 mM
fluorescence
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for fluorescence
storage temp.
2-8°C
Quality Level
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Dieser Artikel | 10236276001 | 268298 | MBD0020 |
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability suitable for nucleic acid staining |
| grade for molecular biology | grade - | grade - | grade - |
| technique(s) transfection: suitable | technique(s) - | technique(s) - | technique(s) DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable |
| fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex ~359 nm, λem ~461 nm | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) |
| form powder | form powder | form solid | form liquid |
| solubility H2O: 20 mg/mL, PBS: insoluble | solubility - | solubility water: 5 mg/mL, ethanol: soluble, methanol: soluble | solubility - |
Application
DAPI ist um ein Vielfaches empfindlicher als Ethidiumbromid zur DNA-Färbung in Agarosegelen. Es kann für sogenanntes Photo-Footprinting von DNA verwendet werden, zum Nachweis gebundener Sonden in Blotting-Anwendungen durch gezielte Visualisierung des doppelsträngigen Komplexes, als auch um Änderungen in der DNA zu untersuchen und um den DNA-Gehalt während der Apoptose mittels Durchflusszytometrie zu bestimmen. DAPI-Färbung ist eine sensitive und spezifische Nachweismethode für Mykoplasmen.
DAPI ist um ein Vielfaches sensitiver als Ethidiumbromid für die Färbung von DNA in Agarosegelen.
Geeignet für
DAPI wird verwendet:
- DNA-Färbung in Agarosegelen
- Analyse von DNA-Veränderungen während der Apoptose
- Nachweis von Mykoplasmen
- Photo-Footprinting von DNA
- Immunfluoreszenzfärbung von Zellen
DAPI wird verwendet:
- zur schnellen Überwachung von mikrobiologischen Kontaminationen[1]
- bei der Chromosomenbänderungstechnik[2]
- zum Nachweis von apoptotischen Zellen[3]
- bei der Fluoreszenzmikroskopie zum Nachverfolgen der DisA (DNA-Integritäts-Scan-Protein)-Bewegung auf Bacillus subtilis-DNA[4]
- zum Färben von ausgereiften Pollenkörnern (0,5 mg/ml)[5]
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Disclaimer
vor Licht schützen
General description
DAPI (4′,6-Diamidino-2-phenylindoldihydrochlorid) ist ein zelldurchdringender, fluoreszierender Farbstoff, der an DNA bindet.
Möglicherweise ist Wärme oder eine Ultraschallbehandlung erforderlich. Bei Lagerung bei Raumtemperatur oder bei 4 °C im Dunkeln sollten die Lösungen 2 bis 3 Wochen lang stabil sein.
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target_organs
Respiratory system
Lagerklasse
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wgk
WGK 3
flash_point_f
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flash_point_c
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ppe
Eyeshields, Gloves, type N95 (US)
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Male karyotype and meiosis of Tenagobia fuscata (Corixoidea, Micronectidae) are studied. The species possesses a male diploid chromosome number 2n = 28 + XY, holokinetic chromosomes, absence of m chromosomes and an achiasmatic male meiosis. Autosomes divide pre-reductionally while the
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J Kapuscinski
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 70(5), 220-233 (1995-09-01)
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and
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As a strategy for the identification of T-DNA-tagged gametophytic mutants, we have used T-DNA insertional mutagenesis based on screening for distorted segregation ratios by antibiotic selection. Screening of approximately 1000 transgenic Arabidopsis families led to the isolation of eight lines
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Can this product be used to stain bacteria (E.coli) chromosome?
1 Antwort-
Hilfreich?
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What is the working dilution of DAPI that should be used to stain the nuclei of human cells?
1 Antwort-
Typical working concentration of DAPI used in most applications is 0.1μg/mL-10μg/mL. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/774/984/d9564pis.pdfPlease see the link below to review a sample protocol from Cold Spring Harbor:
https://cshprotocols.cshlp.org/content/2011/1/pdb.prot5556.longHilfreich?
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what kind of the water used to dissolve DAPI? and what's the temperature should be dissolve the water?
1 Antwort-
This product is soluble at 20 mg/mL in water at ambient temperature. No specific water type or treatment is required. Deionized water is typically used for laboratory applications.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfHilfreich?
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Can the stain concentration be used for staining fish scales and any animal cells for ICC?
1 Antwort-
Yes, DAPI can be used to stain fish scale nuclei. While specific data regarding the concentration and duration for staining fish scale nuclei is not available, it is suggested to start with a working concentration of around 0.5 mg/ml and optimize the staining for each specific cell type, as different cells may uptake the dye at varying rates. It is also advisable to try staining at both higher and lower concentrations. For staining mammalian cells, lower concentrations are typically used.
Hilfreich?
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What is the suggested concentration for product D9542-1MG?
1 Antwort-
The suggested concentration for product D9542-1MG, DAPI, is in the range of 0.5 to 5 ug/ml.
Hilfreich?
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What is the suitable DAPI for immunofluorescence staining of free-floating rat brain sections?
1 Antwort-
Several DAPI products are suitable for staining free-floating rat brain sections. The same DAPI used for staining mycoplasma, cultured cells, or other cell types can be used for this purpose. Any of the DAPI products listed on the website should be suitable for this use.
Hilfreich?
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肝細胞を染める染色プロトコルを教えてください。
1 Antwort-
Please see the link below to review the product datasheet, which includes basic protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfA protocol specifically for hepatocyte staining is not available. However, the publication shown at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671579/Hilfreich?
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Aside from the applications described on the product page, how is D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), used?
1 Antwort-
DAPI is used as a nuclear counterstain in our FISH protocol at 2 μg/mL in antifade mounting media. DAPI can also be used as a vital dye to stain mature pollen grains (0.5 μg/mL). DAPI can bind to RNA, but its selectivity for DNA over RNA is high. It appears that DAPI uses an intercalating binding mode with RNA that is AU selective (Biochem., 31, 3103 (1992)). Selectivity of DAPI for DNA over RNA is greater than that shown by ethidium bromide and propidium iodide (Nucleic Acid Research, 6, 3535 (1979))
Hilfreich?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be dissolved?
1 Antwort-
This product is soluble in water at 20 mg/mL, but may require heat. DAPI does not dissolve readily in PBS, 0.1 M phosphate, or 0.1 M Tris (pH 7).
Hilfreich?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be stored once in solution, and for how long would it be good?
1 Antwort-
Solutions stored in the dark at room temperature have been stable for 2-3 weeks, but solutions exposed to light for the same time period have shown considerable degradation. At 1 mg/mL, solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.
Hilfreich?
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