UHPLC-MS/MS Analysis of Multiple Mycotoxins on Titan C18

column

Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)

mobile phase

[A] 5mm ammonium formate with 0.1% formic acid in water ; [B] 5mm ammonium formate with 0.1% formic acid in acetonitrile

gradient

30% to 60%B in 3 min, to 100%B in 2 min, hold at 100%B for 2 min, 100%B to 30%B in 0.5 min, hold at 30% for 2 min

flow rate

0.4 mL/min

pressure

8557 psi (590 bar)

column temp.

40 °C

detector

MS/MS, ESI(+), MRM

injection

2 μL