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Merck

08-115

Rat Collagen Type I

from rat tail, liquid, 2-5 mg/mL, suitable for cell culture, used for 3D gel formation

Sinonimo/i:

Collagene di coda di topo, Collagene di tipo I di coda di topo

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100 MG

352,00 €

352,00 €

Spedizione prevista il09 dicembre 2025Dettagli

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Informazioni su questo articolo

Codice UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.75

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Nome del prodotto

Collagen Type I, rat tail, 100 mg, solution, BioReagent, suitable for cell culture

Origine biologica

rat

Livello qualitativo

100

Stato

liquid
solution

Produttore/marchio commerciale

Upstate®

Concentrazione

100 mg

tecniche

cell culture | mammalian: suitable (and for 3D proliferation assays.)

input

sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)

N° accesso NCBI

NM_000088.3

N° accesso UniProt

P02452

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Informazioni sul gene

rat ... COL1A1(29393)

Categorie correlate

Descrizione generale

In humans, type I collagen is the most abundant and broadly expressed collagen. This heterotrimer contains two alpha 1 (I) chains and one alpha 2 (I) chain.[1] αα[2] It has repeating Gly-Xaa-Yaa tri-peptides motifs. The collagen alpha-1(I) chain protein is a member of the collagen complex superfamily.[1] COL1A1 has a von Willebrand factor type C (vWFC) domain, a collagen triple-helix repeat, and a fibrillar collagen C-terminal domain (COLF).[2]

Applicazioni



Azioni biochim/fisiol

α It is involved in carboplatin-resistant ovarian cancer (OV).[1] COL1A1 serves as an oncogene in the development of colorectal cancer (CRC).[2] Dominant inherited COL1A1 gene mutations result in connective tissue disorders such as osteogenesis imperfecta (OI).[1]

Stato fisico

0.02N acetic acid, pH 3.67

Nota sulla preparazione

1 anno a 4°C

Risultati analitici

Routinely evaluated by testing for uniform gelation and for attachment of and spreading of PC-12 rat cells

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Salvo diversa indicazione nel nostro catalogo o in altra documentazione fornita dall′azienda insieme al prodotto, i nostri prodotti sono destinati esclusivamente a scopi di ricerca e non devono essere utilizzati per altre finalità, inclusi a titolo esemplificativo ma non esaustivo, fini commerciali non autorizzati, applicazioni diagnostiche in vitro, usi terapeutici ex vivo o in vivo o qualsiasi altro tipo di assunzione o applicazione rivolta agli esseri umani o agli animali.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Kerim B Kaylan et al.
Scientific reports, 6, 23490-23490 (2016-03-31)
The bipotential differentiation of liver progenitor cells underlies liver development and bile duct formation as well as liver regeneration and disease. TGFβ and Notch signaling are known to play important roles in the liver progenitor specification process and tissue morphogenesis.
Localization of matrix metalloproteinase MMP-2 to the surface of invasive cells by interaction with integrin alpha v beta 3
Brooks, P. C., et al
Cell, 85, 683-693 (1996)
Reconstituted rattail collagen used as substrate for tissue cultures on coverslips in Maximow slides and roller tubes.
BORNSTEIN, M B
Laboratory Investigation; a Journal of Technical Methods and Pathology, 7, 134-137 null
M Yebra et al.
The Journal of biological chemistry, 271(46), 29393-29399 (1996-11-15)
The urokinase plasminogen activator (uPA) interacts with its cell surface receptor (uPAR), providing an inducible, localized cell surface proteolytic activity, thereby promoting cellular invasion. Evidence is provided for a novel function of cell surface-associated uPA.uPAR. Specifically, induction of cell surface
S Attoub et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(14), 2329-2338 (2000-10-29)
Leptin plays a key role regulating food intake, body weight and fat mass. These critical parameters are associated with an increased risk for digestive and mammary gland cancer in the Western population. Here we determined whether leptin contributes to the
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Domande

1–5 di 5 domande  

  1. Does this product require pH neutralization by addition of acid or base for gelation?

    1 risposta

    1. The gelation process for this product does not require pH neutralization by the addition of acid or base. The procedure involves pipetting undiluted collagen into culture dishes, exposing them to ammonia vapor, and rinsing with sterile distilled water multiple times to remove ammonia. The final rinse is replaced with a sterile medium or balanced salt solution with serum, and the dishes are stored at 4°C until use.

      Utile?

  2. Hi team: I hope this finds you well. May I know the concentration of collagen type I , rat tail 08-115? Much appreciated. Kind regards, Ovi

    1 risposta

    1. The concentration is provided in lot specific Certificates of Analysis, typically within the range of 3 to 5 mg/ml.

      Utile?

  3. Accidentally, I opened the collagen out of the flow chamber. Should I filter it or autoclave it to ensure the sterile conditions?

    1 risposta

    1. Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution.
      Avoid sterilizing the collagen solution by membrane filtration, as substantial protein loss may occur by this method. The coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes may then be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol.

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  4. Hi, what is the concentration of the collagen?

    1 risposta

    1. The concentration of this product will be listed on the lot-specific Certificate of Analysis. Please see the link below to access a Certificate:
      https://www.emdmillipore.com/US/en/product/Collagen-Type-I-rat-tail,MM_NF-08-115#anchor_COA

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  5. Once diluted in 0.02N acetic acid, does the collagen solution require sterilisation for use in cell culture? If so, which method is the most effective: UV, autoclave or 0.22uM sterile filter (should this be PES or PVDF)?

    1 risposta

    1. This product is supplied as a sterile solution in 0.02 N Acetic acid. The package size is 100 mg, however, the concentration is variable and reported on the lot-specific Certificate of Analysis. The typical range is 4 - 5 mg/mL. Dilutions can be made with sterile PBS. Further sterilization is not required. If necessary, coated slides and coverslips can be sterilized by UV.

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