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C3142
α-Chymotrypsin from bovine pancreas
(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein
Sinonimo/i:
TLCK-Chymotrypsin
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Cambia visualizzazione
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
10 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 103,00 € |
25 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 173,00 € |
100 mg | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 517,00 € |
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
eCl@ss:
42010112
EC Number:
232-671-2
NACRES:
NA.54
MDL number:
Numero CE:
Specific activity:
≥40 units/mg protein
Assay:
≥90% (UV)
103,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartiQuality Segment
type
Type VII
assay
≥90% (UV)
form
essentially salt-free, lyophilized powder
specific activity
≥40 units/mg protein
mol wt
25 kDa
solubility
1 mM HCl: soluble 10 mg/mL, clear
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... CTRB1(618826)
Application
Biochem/physiol Actions
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu2+ and Hg2+.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
Preparation Note
TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.
Analysis Note
Protein determined by A1%/280
Other Notes
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
1 of 1
Questo articolo | |||
|---|---|---|---|
| description (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein | description Type I-S, essentially salt-free, lyophilized powder | description suitable for protein sequencing, salt-free, lyophilized powder | description Type II, lyophilized powder, ≥40 units/mg protein |
| Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) |
| specific activity ≥40 units/mg protein | specific activity ≥40 units/mg protein | specific activity - | specific activity ≥40 units/mg protein |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form salt-free, lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| mol wt 25 kDa | mol wt 25 kDa | mol wt 25 kDa | mol wt 25 kDa |
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Parola di avvertenza
Danger
Codici di pericolo
Avvertenze di sicurezza
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Classe di stoccaggio
11 - Combustible Solids
WGK
WGK 1
DPI (dispositivi di protezione individuale)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Elenchi normativi
Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.
9004-07-3
CAS No.
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What is the Department of Transportation shipping information for this product?
1 risposta-
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How can solutions of alpha-chymotrypsin (Product No. C3142) be stored?
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Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.
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What is the pH optimum for the enzyme, Product No. C3142?
1 risposta-
The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).
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What is the molecular weight of alpha-chymotrypsin from bovine pancreas (Product No. C3142)?
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The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.This information and the basic structure description of the enzyme can be found on our product information sheet (under Documents, above) at our website.
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How do you recommend preparing a solution of Product No. C3142?
1 risposta-
It is recommended to reconstitute Product No. C3142 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as both a stabilizer (and possibly an activator) of the enzyme.
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