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Merck

CHOGS

CHOZN® ZFN-Modified CHO Cell Line

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Informazioni su questo articolo

Codice UNSPSC:
12352200
NACRES:
NA.75

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Descrizione

CHOZN® GS-/- ZFN-modified CHO cell line
glutamine synthetase knockout cell line

Solubilità

DMSO: 5 mg/mL, clear

Condizioni di spedizione

liquid nitrogen

Temperatura di conservazione

−196°C

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Categorie correlate

Descrizione generale

CHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion media
ZFNs are a class of engineered DNA-binding proteins and causes a double-strand break. This double-strand break is necessary for site-specific mutagenesis mediated DNA repair. It facilitates targeted genome editing by binding to a user-specified locus.[1] Glutamine synthetase (GS) is one of the most commonly used selectable markers in the biopharmaceutical industry. Glutamine is an essential amino acid for cellular growth.[2] The GS enzyme is responsible for the conversion of glutamate into glutamine.[3] Without this enzymatic activity, cells can no longer synthesize glutamine endogenously and if glutamine is not supplemented in the culture media, the cells will die.

Azioni biochim/fisiol

CHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.

Confezionamento

This product is shipped in liquid nitrogen dry vapor shippers.
Liquid nitrogen dry vapor shipper fee (up to a maximum of $125) and freight not included in price of product.
Liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. A $450 liquid nitrogen dry vapor shipper fee will be charged if not returned within 1 week of delivery.
Learn more about liquid nitrogen dry vapor shipper

Stato fisico

CHOZN cell lines are provided to customers in vials containing >7.5e6 cells/mL

Note legali

All CHOZN cell lines are sold under license from Sigma Aldrich. A copy of the research license is provided with purchase of the CHOZN cell lines.
CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
2113545207
2113545207
2113-53667
051M0924

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Q's next: the diverse functions of glutamine in metabolism, cell biology and cancer.
DeBerardinis R J and Cheng T
Oncogene, 29(3), 313-313 (2010)
Cytotoxic effects during knock out of multiple porcine endogenous retrovirus (PERV) sequences in the pig genome by zinc finger nucleases (ZFN).
Semaan M, et al.
PLoS ONE, 10(4), e0122059-e0122059 (2015)
The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type.
Yuneva M O, et al.
Cell Metabolism, 15(2), 157-170 (2012)

Articoli

CHO Media and Feeds for Optimized Production of Therapeutic Proteins

Cellvento® and EX-CELL® CHO cell culture media and feeds, optimized for monoclonal antibody & recombinant protein production in fed-batch & perfusion

Optimizing CHO Cells for Higher Productivity

Cellvento 4CHO-X Expansion Medium enhances cell biomass for bioreactor inoculation in cell expansion stages.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

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Contenuto correlato

Accelerate development and optimization of cell culture media for upstream perfusion processes

This page describes the use of a small-scale perfusion bioreactor with integrated microfluidic flow control and sensor technologies to predict large-scale processes.

Glycosylation Modulation in Fed-batch Production of Therapeutic Proteins

Enhance mAb and recombinant protein performance, efficacy, and stability with precise glycosylation modulation using the Cellvento® ModiFeed platform.

Cell Line Development for mAbs

Learn more about cell line development and the relevant products you need for this process.

User Guide CHOZN GS Platform

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