F3385

Fetuin from fetal bovine serum

lyophilized powder, BioReagent, suitable for cell culture

• Sinonimo/i: Fetuin from fetal calf serum
• Numero CAS: 9014-81-7
• UNSPSC Code: 12352202
• EC Number: 232-762-7
• NACRES: NA.75
• MDL number: MFCD00131055
• Form: lyophilized powder
• Mol wt: 48.4 kDa

Proprietà

• product line: BioReagent
• Quality Segment: 200
• form: lyophilized powder
• mol wt: 48.4 kDa
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.3% free N-acetylneuraminic acid
• solubility: H₂O: 1 mg/mL
• storage temp.: 2-8°C

Descrizione

General description

Fetuin from fetal bovine serum is a plasma glycoprotein. It is a 64 kDa glycoprotein, which is produced by the liver and adipose tissue.
A glycoprotein derived from FBS that is used as Fetuin has been used at a concentration of 500 mg/ml to supplement serum free F12 medium (along with insulin, transferrin and 2-mercaptoethanol) in culture of embryonal carcinoma cells.

Application

Fetuin from fetal bovine serum has been used:

• as a substrate to determine neuraminidase activity
• to validate the specificity of sialidases
• to stabilize m-calpain

Biochem/physiol Actions

Fetuin acts as an inhibitor of insulin receptor tyrosine kinase. It modulates bone remodeling and calcium metabolism. Fetuin regulates vascular calcification, insulin resistance, protease activity control, keratinocytes migration and breast tumor cell proliferative signaling. It functions as a biomarker for neurodegenerative diseases. Fetuin exhibits anti-inflammatory property.

Preparation Note

Prepared by ammonium sulfate fractionation of fetal bovine serum by the method of Pederson, K.O., J. Phys. and Colloid Chem., 51, 164 (1947).

Informazioni sulla sicurezza

• Classe di stoccaggio: 11 - Combustible Solids
• WGK: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable
• DPI (dispositivi di protezione individuale): Eyeshields, Gloves, type N95 (US)