Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.

Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.

Nanomedicine (London, England) (2015-05-27)

Natalia Barkalina, Celine Jones, Helen Townley, Kevin Coward

ABSTRACT

This study aimed to investigate the effects of actively targeting mesoporous silica nanoparticles (MSNPs) toward mammalian sperm with a cell-penetrating peptide (C105Y), with subsequent analysis of binding rates and nano-safety profiles. Boar sperm were exposed in vitro to C105Y-functionalized MSNPs or free C105Y, in a series of increasing doses for up to 2 h, followed by the evaluation of sperm motility, kinematic parameters, acrosome morphology, MSNP-sperm binding and cell fluorescence levels. C105Y-functionalized MSNPs preserved their biocompatibility with sperm, and exhibited an approximately fourfold increase in affinity toward gametes, compared with unmodified MSNPs, during the early stages of incubation. Our findings support the application of MSNPs and active targeting to sperm as valuable tools for reproductive biology.

MATERIALI

Numero di prodotto

Marchio

Descrizione del prodotto

440140

Sigma-Aldrich

(3-amminopropil)trietossisilano, 99%

F8775

Sigma-Aldrich

Formaldeide, Molecular Biology, 36.5-38% in H₂O

72068

Sigma-Aldrich

Idrossido di sodio, BioUltra, Molecular Biology, 10 M in H₂O

H5882

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Esadeciltrimetilammonio bromuro, ≥98%

S2770

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Idrossido di sodio, 1.0 N, BioReagent, suitable for cell culture

H9892

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Acido cloridrico, 1.0 N, BioReagent, suitable for cell culture

252549

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Formaldeide, ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

H6269

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Esadeciltrimetilammonio bromuro, Molecular Biology, ≥99%

284505

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1,2-dicloroetano, anhydrous, 99.8%

H1758

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Acido cloridrico, 36.5-38.0%, BioReagent, Molecular Biology

151173

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Ninhydrin, ACS reagent

2104

Supelco

Acido cloridrico, volumetric, 0.1 M HCl (0.1N), endotoxin free

47608

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Formaldeide, Molecular Biology, BioReagent, ≥36.0% in H₂O (T)

H9151

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Esadeciltrimetilammonio bromuro, BioXtra, ≥99%

741442

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(3-amminopropil)trietossisilano, ≥98.0%

A3648

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(3-amminopropil)trietossisilano, ≥98%

84429

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Acido cloridrico, ~6 M in H₂O, for amino acid analysis

45726

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Acido acetico, suitable for luminescence, BioUltra, ≥99.5% (GC)

15512

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Formaldeide, meets analytical specification of USP, ≥34.5 wt. %

A8380

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5α-Androstan-17β-ol-3-one, ≥97.5%

705551

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Cloruro di idrogeno, 3 M in cyclopentyl methyl ether (CPME)

71687

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Sodio idrossido, BioUltra, suitable for luminescence, ≥98.0% (T), pellets

52365

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Esadeciltrimetilammonio bromuro, BioUltra, Molecular Biology, ≥99.0% (AT)

N4876

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Ninhydrin, suitable for amino acid detection

719803

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