14-840-M

AMPK (α1, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

• 동의어(들): AMPK kinase
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in E. coli
• 양식: liquid
• 유통기한: 6 mo.
• 분자량: M_w 30 kDa (AMPKß1); M_w 38 kDa (AMPKγ1); M_w 64 kDa (AMPKα1)
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable (kinase)
• solubility: water: soluble
• GenBank 수납 번호: AF100763
• NCBI 수납 번호: NM_002733; NM_006253
• UniProt 수납 번호: P54619; Q13131; Q9Y478
• 저장 온도: −70°C
• 유전자 정보: human ... PRKAB1(5564)

설명

일반 설명

Research area: Cell Signaling

Product Source: Expressed in E. coli cells and activated in vivo.

Protein Target: AMPK (α1, β1, γ1)

Target Sub-Family: CAMK

Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full- length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1. The AMPK α catalytic subunit in mammals is encoded by α1 and α2, two β genes β1 and β2 and three γ subunit genes γ1, γ2, and γ3.[1]

애플리케이션

For use in Kinase Assays.

Research Category
Metabolism

Apoptosis & Cancer

Research Sub Category
Diabetes

Obesity

Metabolic Disorders

생화학적/생리학적 작용

Under conditions of nutrient scarcity, AMPK functions as a metabolic checkpoint and inhibits cellular growth.[1] Upon activation, AMPK promotes glucose uptake and fatty acid oxidation thereby reducing energy-consuming processes like protein and lipid synthesis.[2] Furthermore, AMPK regulates autophagy and mitophagy.[3] It also facilitates catabolic pathways to generate more ATP as a response to decreased ATP production.[1]

포장

Also available in 250µg size as catalog # 14-840M - Call for pricing and availability.

물리적 형태

Cobalt-agarose

제조 메모

6 months at -70°C

분석 메모

Routinely evaluated by phosphorylation of substrate peptide

기타 정보

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

법적 정보

GenBank is a registered trademark of United States Department of Health and Human Services

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable