Yes, extending the spin times is sufficient. However, the time range would have to be experimentally determined by the user.
UFC8010
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
동의어(들):
Centrifugal concentrator, 10 kDa MWCO, 4 mL sample volume
다음으로 건너뛰기
제품 이름
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO, sample volume 4 mL, regenerated cellulose membrane, MWCO 10 kDa
material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
4 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL
4 mL
pore size
10 kDa MWCO
Quality Level
greener alternative category
shipped in
ambient
1 of 4
이 품목 | UFC201024 | UFC9010 | UFC5010 |
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| parameter 4 mL sample volume | parameter 2 mL sample volume | parameter 15 mL sample volume | parameter 0.5 mL sample volume |
| material polyethylene cap, polypropylene tube, regenerated cellulose support, polyethylene liner, styrene-butadiene housing, regenerated cellulose membrane | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing |
| pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO | pore size 10 kDa MWCO |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
Application
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
General description
Amicon® Ultra 4 mL centrifugal devices with Ultracel® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
10-13 - German Storage Class 10 to 13
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Filtration methods and membrane filter selection in nanoparticle purification and production.
프로토콜
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
Accurate measurement of specific proteins in urine is important for diagnosing and managing diseases.
Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.
Biomarkers play an essential role in the drug discovery and development process.
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If my centrifuge only goes to 3,052 g, is it okay to centrifuge for a longer period of time to get the same separation? It is a swing-bucket centrifuge using the 10 MWCO filters.
1 답변-
도움이 되었습니까?
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is-it possible to wash and re-use the Amicon@Ultra-4 ? if yes how many time is_it possible to use them ?
1 답변-
The unit is for one-time use only.
도움이 되었습니까?
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How should the tubes be sterilized without damaging the membrane?
1 답변-
There are no validated sterilization methods for the Amicon Ultra devices. However, these devices can be "sanitized" with a spin cycle with 70% ethanol, followed by 2 rinsing spins with sterile water. It is recommended to filter the concentrated sample using a sterile 0.2 µm syringe filter (e.g., SLGVR33RS) after processing on the Amicons.
도움이 되었습니까?
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What speed can this product be centrifuged at ?
1 답변-
For this item when using a swinging-bucket rotor, spin the device at 4,000 × g maximum for approximately 10-40 minutes. When using a fixed-angle rotor, orient the device with the membrane panel facing up. For Amicon® Ultra 3K, 10K, 30K, and 50K devices, spin at 7,500 × g maximum for approximately 10-40 minutes. For Amicon® Ultra 100K devices, spin at 5,000 × g maximum for approximately 10-20 minutes.
도움이 되었습니까?
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How to use it...how much sample should be loaded and what will be the protocol of centrifugation while using?
1 답변-
The maximum initial sample 4 volume (mL). For more information please see page 36 in this product https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/181/br5002en-protein-prep-workflow-ms.pdf
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